PIK3 gamma Protein (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for PIK3 gamma Protein (Myc-DYKDDDDK Tag) (ABIN2728968)
Target
See all PIK3 gamma (PIK3CG) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PIK3 gamma protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PIK3CG protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) (AA 1-1102) protein (His tag)
custom-made PIK3CG Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) (AA 1-1102) protein (His tag)custom-made PIK3CG Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Cilostazol Induces PGI2 Production via Activation of the Downstream Epac-1/Rap1 Signaling Cascade to Increase Intracellular Calcium by PLC? and to Activate p44/42 MAPK in Human Aortic Endothelial ..." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132835, (2015) (PubMed).
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- PIK3 gamma (PIK3CG) (Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG))
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Alternative Name
- Pik3cg
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Background
- Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.
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Molecular Weight
- 126.3 kDa
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NCBI Accession
- NP_002640
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Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Inositol Metabolic Process, Hepatitis C, VEGF Signaling
Target
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