PLK1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729185

This Recombinant PLK1 protein is expressed in HEK-293 Cells.

Quick Overview for PLK1 Protein (Myc-DYKDDDDK Tag) (ABIN2729185)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This PLK1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human PLK1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: Plk1

Background: The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis hence, it is a target for cancer therapy.

Molecular Weight: 68.1 kDa

NCBI Accession: NP_005021

Pathways: Cell Division Cycle, M Phase