POLR2E Protein (Myc-DYKDDDDK Tag)
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- Target See all POLR2E Proteins
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POLR2E protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human POLR2E protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product POLR2E Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- Alternative Name
- Polr2e (POLR2E Products)
- Synonyms
- MGC68604 Protein, polr2e Protein, wu:fk97g10 Protein, zgc:101098 Protein, 2410021N14Rik Protein, 25kDa Protein, AW208866 Protein, RPB5 Protein, XAP4 Protein, RPABC1 Protein, hRPB25 Protein, hsRPB5 Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog Protein, RNA polymerase II subunit E Protein, polymerase (RNA) II (DNA directed) polypeptide E, b Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa Protein, polymerase (RNA) II (DNA directed) polypeptide E Protein, DNA-directed RNA polymerases I, II, and III subunit RPABC1 Protein, polr2e.S Protein, POLR2E Protein, polr2eb Protein, polr2e Protein, Polr2e Protein, rpb-5 Protein
- Background
- This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.
- Molecular Weight
- 24.4 kDa
- NCBI Accession
- NP_002686
- Pathways
- Regulatory RNA Pathways
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