PPP1R12B Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag)
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- Target See all PPP1R12B Proteins
- PPP1R12B (Protein Phosphatase 1, Regulatory Subunit 12B (PPP1R12B))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 4
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PPP1R12B protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PPP1R12B / MYPT2 (transcript variant 4) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PPP1R12B Protein
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Protein Phosphatase 1, Regulatory Subunit 12B (PPP1R12B) (AA 1-386) protein (GST tag)
PPP1R12B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protein Phosphatase 1, Regulatory Subunit 12B (PPP1R12B) (AA 1-982) protein (Strep Tag)Crystallography grade PPP1R12B Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Protein Phosphatase 1, Regulatory Subunit 12B (PPP1R12B) (AA 1-976) protein (Strep Tag)Crystallography grade PPP1R12B Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PPP1R12B (Protein Phosphatase 1, Regulatory Subunit 12B (PPP1R12B))
- Alternative Name
- Ppp1r12b,mypt2 (PPP1R12B Products)
- Synonyms
- 1810037O03Rik Protein, 9530009M10Rik Protein, AI132431 Protein, Mypt2 Protein, MYPT2 Protein, PP1bp55 Protein, protein phosphatase 1, regulatory subunit 12B Protein, protein phosphatase 1, regulatory (inhibitor) subunit 12B Protein, protein phosphatase 1 regulatory subunit 12B Protein, Ppp1r12b Protein, PPP1R12B Protein
- Background
- Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome.
- Molecular Weight
- 26.1 kDa
- NCBI Accession
- NP_115287
- Pathways
- M Phase
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