PRAME Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag)
Quick Overview for PRAME Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag) (ABIN2729549)
Target
See all PRAME ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 4
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Purification tag / Conjugate
- This PRAME protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PRAME / MAPE (transcript variant 4) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (His tag)
custom-made PRAME Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (GST tag)PRAME Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 321-420) protein (His-IF2DI Tag)PRAME Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509), (full length) protein (His tag)PRAME Origin: Human Host: Yeast Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
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Alternative Name
- Prame,mape
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Background
- This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 57.7 kDa
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NCBI Accession
- NP_996838
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Pathways
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
Target
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