RAB8A Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730373

This Recombinant RAB8A protein is expressed in HEK-293 Cells.

Quick Overview for RAB8A Protein (Myc-DYKDDDDK Tag) (ABIN2730373)

Target: RAB8A (RAB8A, Member RAS Oncogene Family (RAB8A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This RAB8A protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human RAB8A protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RAB8A (RAB8A, Member RAS Oncogene Family (RAB8A))

Alternative Name: Rab8a

Background: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97 % , 96 %, and 51 % similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.

Molecular Weight: 23.5 kDa

NCBI Accession: NP_005361

Pathways: M Phase, Regulation of long-term Neuronal Synaptic Plasticity, SARS-CoV-2 Protein Interactome