Splicing Factor 1 Protein (SF1) (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2732606

Product Details

Target: Splicing Factor 1 (SF1)

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 3

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Splicing Factor 1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Splicing factor 1 (SF1) (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Splicing Factor 1 (SF1)

Abstract: SF1 Products

Synonyms: BBP Protein, D11S636 Protein, MBBP Protein, ZCCHC25 Protein, ZFM1 Protein, ZNF162 Protein, BB094781 Protein, CW17R Protein, MZFM Protein, WBP4 Protein, Zfp162 Protein, CG5836 Protein, Dmel\\CG5836 Protein, cg5836 Protein, dSF1 Protein, p70 Protein, SF1 Protein, sf1 Protein, wu:fc09f06 Protein, znf162 Protein, MWD22.25 Protein, MWD22_25 Protein, SF-1 Protein, splicing factor 1 Protein, Splicing factor 1 Protein, splicing factor-like protein Protein, nuclear receptor subfamily 5 group A member 1 Protein, splicing factor 1 S homeolog Protein, SF1 Protein, Sf1 Protein, sf1 Protein, AT5G51300 Protein, NR5A1 Protein, sf1.S Protein

Background: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3&apos splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Molecular Weight: 59.5 kDa

NCBI Accession: NP_973727

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location