Thrombopoietin Protein (THPO) (Transcript Variant 1)

Catalog No. ABIN2733599

Product Details

Target: Thrombopoietin (THPO)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Protein Interaction (PI), Standard (STD), Antibody Production (AbP)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Thrombopoietin (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin (THPO Products)

Synonyms: MGDF Protein, MKCSF Protein, ML Protein, MPLLG Protein, THCYT1 Protein, TPO Protein, Mgdf Protein, Ml Protein, Mpllg Protein, Tpo Protein, Tpo1 Protein, Tpo2 Protein, Tpo3 Protein, Tpo4 Protein, tpo Protein, LOC100231762 Protein, thrombopoietin Protein, THPO Protein, Thpo Protein, thpo Protein

Background: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 18.6 kDa

NCBI Accession: NP_000451

Pathways: JAK-STAT Signaling, Hormone Activity