NADK Protein (AA 64-446, Catalytic Domain) (His tag)
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- Target See all NADK Proteins
- NADK (NAD Kinase (NADK))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Catalytic Domain, AA 64-446
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NADK protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Human
- Characteristics
- Human NAD kinase (aa 64-446) is fused at the N-terminus to a His-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- Free of any nucleotide converting side-activities.
- Top Product
- Discover our top product NADK Protein
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NAD Kinase (NADK) (AA 64-298) protein (His tag)
NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-446) protein (Strep Tag)Crystallography grade NADK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
NAD Kinase (NADK) (AA 1-439) protein (Strep Tag)Crystallography grade NADK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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>2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol of NADP per min.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Prepare working aliquots by centrifugation and dissolve the pellet in 50 mM TRIS/HCl pH 7.5, 150 mM sodium chloride and 1 mM DTT.
- Concentration
- Lot specific
- Buffer
- Ammonium sulphate suspension.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.
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- Target
- NADK (NAD Kinase (NADK))
- Alternative Name
- NAD Kinase (NADK Products)
- Synonyms
- 4432404C02Rik Protein, BC004012 Protein, dJ283E3.1 Protein, NAD kinase Protein, Nadk Protein, NADK Protein
- Background
- NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.
- Molecular Weight
- ~42kDa (monomer)
- UniProt
- O95544
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