Angiopoietin 1 Protein (ANGPT1) (AA 20-498)

Catalog No. ABIN2816939

This Recombinant Angiopoietin 1 protein is expressed in Mammalian Cells.

Quick Overview for Angiopoietin 1 Protein (ANGPT1) (AA 20-498) (ABIN2816939)

Target: Angiopoietin 1 (ANGPT1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Functional Studies (Func)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 20-498

Sequence: Ser20 - Phe498, Accession # Q5HYA0

Characteristics: Biological Activity: The ED50, as determined by inhibiting serum deprivation induced apoptosis in human HUVEC, is less than 50 ng/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody.

Sterility: 0.2 μm filtered

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris-Citrate, pH 6.5, 500 mM NaCl

Storage: -20 °C

Target Details

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: ANGPT1

Background: Relevance: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. {ECO:0000269|PubMed:15284220, ECO:0000269|PubMed:18425119, ECO:0000269|PubMed:18425120, ECO:0000269|PubMed:9204896}.
Synonyms: ANG-1,

Molecular Weight: 56.3 kDa

UniProt: Q15389

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process