PHPT1 Protein (His tag)
Quick Overview for PHPT1 Protein (His tag) (ABIN3011151)
Target
See all PHPT1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PHPT1 protein is labelled with His tag.
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Purpose
- Mouse Phosphohistidine Phosphatase 1 (PHPT1) Protein
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Sequence
- MAADLGQIPD VDIDSDGVFK YVLIRVHLAE PSGDPAKECK EIVRGYKWAE YHADIYDKVS GELQRNGYDC ECLGGGRISH QSQDRKIHVY GYSMGYGRAQ HSVSTEKIKA KYPDYEVTWA DDGY
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Biological Activity Comment
- Not tested
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phosphohistidine Phosphatase 1 (PHPT1) (AA 1-124) protein (His tag)
custom-made PHPT1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125) protein (His-IF2DI Tag)PHPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125) (Active) protein (His tag)PHPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125) protein (GST tag)PHPT1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, PAA, WB
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125), (full length) protein (GST tag)PHPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
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Alternative Name
- Phosphohistidine Phosphatase 1
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Molecular Weight
- Calculated MW: 15.5 kDa
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UniProt
- Q9DAK9
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Pathways
- Positive Regulation of Peptide Hormone Secretion
Target
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