PHPT1 Protein (His tag)

Catalog No. ABIN7317373

Product Details

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PHPT1 protein is labelled with His tag.

Purpose: Recombinant Human PHPT1/PHP14 Protein (His Tag)

Sequence: Ala 2-Tyr 125

Characteristics: A DNA sequence encoding the human PHPT1 (Q9NRX4-1) (Ala 2-Tyr 125) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 97 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Alternative Name: PHPT1/PHP14 (PHPT1 Products)

Synonyms: wu:fb50e08 Protein, zgc:101086 Protein, MGC131298 Protein, PHP14 Protein, CGI-202 Protein, HSPC141 Protein, RP11-216L13.10 Protein, bA216L13.10 Protein, 1700008C22Rik Protein, Php14 Protein, phosphohistidine phosphatase 1 Protein, phosphohistidine phosphatase 1 L homeolog Protein, 14 kDa phosphohistidine phosphatase-like Protein, LOC424401 Protein, phpt1 Protein, PHPT1 Protein, phpt1.L Protein, LOC100511240 Protein, Phpt1 Protein

Background:

Background: PHPT1, also known as 14 kDa phosphohistidine phosphatase, phosphohistidine phosphatase 1, protein janus-A homolog, PHP14, is a cytoplasm protein which belongs to the janus family. PHPT1 / PHP14 is expressed abundantly in heart and skeletal muscle. Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells. The knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of human PHPT1 have been solved. PHPT1 / PHP14 can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues.

Synonym: CGI-202;HEL-S-132P;HSPC141;PHP14

Molecular Weight: 15.2 kDa

Pathways: Positive Regulation of Peptide Hormone Secretion