Protease protein (His tag)

Catalog No. ABIN3011321

This Recombinant Protease protein is expressed in Escherichia coli (E. coli).

Quick Overview for Protease protein (His tag) (ABIN3011321)

Target: Protease

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: > 90 %

Product Details

Purification tag / Conjugate: His tag

Purpose: Human Serine Protease 1 (PRSS1) Protein

Sequence: SLINEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPQYD RKTLNNDIML IKLSSRAVIN ARVSTISLPT APPATGTKCL ISGWGNTASS GADYPDELQC LDAPVLSQAK CEASYPGKIT SNMFCVGFLE GGKDSCQGDS GGPVVCNGQL QGVVSWGDGC AQKNKPGV

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 1 mM EDTA, 5 % Trehalose.

Preservative: Other preservative

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: Protease

Target Type: Viral Protein

Molecular Weight: Calculated MW: 22.7 kDa
Observed MW (SDS-PAGE): 26 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

Gene ID: 5644

HGNC: 9475

OMIM: 167800

UniProt: P07477