Protease protein (His tag)

Catalog No. ABIN3011322

Recombinant Protease protein expressed in Escherichia coli (E. coli).

Quick Overview for Protease protein (His tag) (ABIN3011322)

Target: Protease

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: > 95 %

Product Details

Purification tag / Conjugate: His tag

Purpose: Mouse Protease, Serine 12 (PRSS12) Protein

Sequence: GYCDCGQG PALPVIRLVG GNSGHEGRVE LYHAGQWGTI CDDQWDNADA DVICRQLGLS GIAKAWHQAH FGEGSGPILL DEVRCTGNEL SIEQCPKSSW GEHNCGHKED AGVSCVPLTD GVIRLAGGKS THEGRLEVYY KGQWGTVCDD GWTEMNTYVA CRLLGFKYGK QSSVNHFDGS NRPIWLDDVS CSGKEVSFIQ CSRRQWGRHD CSHREDVGLT CYPDSDGHRL SPGFPIRLVD GENKKEGRV

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: Protease

Target Type: Viral Protein

Molecular Weight: Calculated MW: 30.9 kDa

UniProt: O08762