GPR177/WLS Protein (AA 43-232) (His tag)
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- Target See all GPR177/WLS (WLS) Proteins
- GPR177/WLS (WLS) (Wntless Homolog (WLS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 43-232
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GPR177/WLS protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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VSYMSVKCVD ARKNHHKTKW FVPWGPNHCD KIRDIEEAIP REIEANDIVF SVHIPLPHME MSPWFQFMLF ILQLDIAFKL NNQIRENAEV SMDVSLAYRD DAFAEWTEMA HERVPRKLKC TFTSPKTPEH EGRYYECDVL PFMEIGSVAH KFYLLNIRLP VNEKKKINVG IGEIKDIRLV GIHQNGGFTK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human WLS Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
- Top Product
- Discover our top product WLS Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- GPR177/WLS (WLS) (Wntless Homolog (WLS))
- Alternative Name
- WLS (WLS Products)
- Synonyms
- C1orf139 Protein, EVI Protein, GPR177 Protein, MRP Protein, mig-14 Protein, 5031439A09Rik Protein, AI173978 Protein, AI987742 Protein, Gpr177 Protein, gpr117 Protein, gpr177 Protein, sb:cb610 Protein, wu:fb36d04 Protein, wu:fb44d10 Protein, wu:fb50c06 Protein, zgc:64091 Protein, CG6210 Protein, Dmel\\CG6210 Protein, Evi Protein, Evi/Wls Protein, Srt Protein, Wls Protein, Wls/Evi Protein, evi Protein, srt Protein, wls/evi Protein, wntless Wnt ligand secretion mediator Protein, wntless homolog (Drosophila) Protein, wntless Protein, WLS Protein, Wls Protein, wls Protein
- Background
- Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity). {ECO:0000250, ECO:0000269|PubMed:16678095, ECO:0000269|PubMed:16678096}.
- Molecular Weight
- 23.2 kDa Including tag.
- UniProt
- Q5T9L3
- Pathways
- WNT Signaling
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