ZC3H11A Protein (AA 1-810) (Strep Tag)

Catalog No. ABIN3110605

Product Details

Target: ZC3H11A (Zinc Finger CCCH-Type Containing 11A (ZC3H11A))

Protein Type: Recombinant

Protein Characteristics: AA 1-810

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ZC3H11A protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MPNQGEDCYF FFYSTCTKGD SCPFRHCEAA IGNETVCTLW QEGRCFRQVC RFRHMEIDKK RSEIPCYWEN QPTGCQKLNC AFHHNRGRYV DGLFLPPSKT VLPTVPESPE EEVKASQLSV QQNKLSVQSN PSPQLRSVMK VESSENVPSP THPPVVINAA DDDEDDDDQF SEEGDETKTP TLQPTPEVHN GLRVTSVRKP AVNIKQGECL NFGIKTLEEI KSKKMKEKSK KQGEGSSGVS SLLLHPEPVP GPEKENVRTV VRTVTLSTKQ GEEPLVRLSL TERLGKRKFS AGGDSDPPLK RSLAQRLGKK VEAPETNIDK TPKKAQVSKS LKERLGMSAD PDNEDATDKV NKVGEIHVKT LEEILLERAS QKRGELQTKL KTEGPSKTDD STSGARSSST IRIKTFSEVL AEKKHRQQEA ERQKSKKDTT CIKLKIDSEI KKTVVLPPIV ASRGQSEEPA GKTKSMQEVH IKTLEEIKLE KALRVQQSSE SSTSSPSQHE ATPGARRLLR ITKRTGMKEE KNLQEGNEVD SQSSIRTEAK EASGETTGVD ITKIQVKRCE TMREKHMQKQ QEREKSVLTP LRGDVASCNT QVAEKPVLTA VPGITRHLTK RLPTKSSQKV EVETSGIGDS LLNVKCAAQT LEKRGKAKPK VNVKPSVVKV VSSPKLAPKR KAVEMHAAVI AAVKPLSSSS VLQEPPAKKA AVAVVPLVSE DKSVTVPEAE NPRDSLVLPP TQSSSDSSPP EVSGPSSSQM SMKTRRLSSA STGKPPLSVE DDFEKLIWEI SGGKLEAEID LDPGKDEDDL LLELSEMIDS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ZC3H11A (Zinc Finger CCCH-Type Containing 11A (ZC3H11A))

Alternative Name: ZC3H11A (ZC3H11A Products)

Synonyms: ZC3HDC11A Protein, 1110003F06Rik Protein, 5730454B08Rik Protein, G630041M05Rik Protein, Zc3hdc11a Protein, Zc3hh11a Protein, mKIAA0663 Protein, RGD1308290 Protein, ZC3H11B Protein, MGC142959 Protein, fc32e10 Protein, wu:fc32e10 Protein, zgc:73328 Protein, zinc finger CCCH-type containing 11A Protein, zinc finger CCCH type containing 11A Protein, zinc finger CCCH-type containing 11A S homeolog Protein, ZC3H11A Protein, Zc3h11a Protein, zc3h11a Protein, zc3h11a.S Protein

Background: Zinc finger CCCH domain-containing protein 11A,FUNCTION: RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export, probably mediated by association with the TREX complex. {ECO:0000269|PubMed:22928037, ECO:0000269|PubMed:29610341}., FUNCTION: (Microbial infection) Plays a role in efficient growth of several nuclear-replicating viruses such as HIV-1, influenza virus or herpes simplex virus 1/HHV-1. Required for efficient viral mRNA Export. {ECO:0000269|PubMed:29610341}.

Molecular Weight: 89.1 kDa

UniProt: O75152