ZNF703 Protein (AA 2-590) (His tag)
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- Target See all ZNF703 Proteins
- ZNF703 (Zinc Finger Protein 703 (ZNF703))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-590
- Origin
- Human
- Source
- Insect Cells
- Purification tag / Conjugate
- This ZNF703 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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SDSPAGSNPR TPESSGSGSG GGGKRPAVPA AVSLLPPADP LRQANRLPIR VLKMLSAHTG HLLHPEYLQP LSSTPVSPIE LDAKKSPLAL LAQTCSQIGK PDPPPSSKLN SVAAAANGLG AEKDPGRSAP GAASAAAALK QLGDSPAEDK SSFKPYSKGS GGGDSRKDSG SSSVSSTSSS SSSSPGDKAG FRVPSAACPP FPPHGAPVSA SSSSSSPGGS RGGSPHHSDC KNGGGVGGGE LDKKDQEPKP SPEPAAVSRG GGGEPGAHGG AESGASGRKS EPPSALVGAG HVAPVSPYKP GHSVFPLPPS SIGYHGSIVG AYAGYPSQFV PGLDPSKSGL VGGQLSGGLG LPPGKPPSSS PLTGASPPSF LQGLCRDPYC LGGYHGASHL GGSSCSTCSA HDPAGPSLKA GGYPLVYPGH PLQPAALSSS AAQAALPGHP LYTYGFMLQN EPLPHSCNWV AASGPCDKRF ATSEELLSHL RTHTALPGAE KLLAAYPGAS GLGSAAAAAA AAASCHLHLP PPAAPGSPGS LSLRNPHTLG LSRYHPYGKS HLSTAGGLAV PSLPTAGPYY SPYALYGQRL ASASALGYQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ZNF703 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ZNF703 (Zinc Finger Protein 703 (ZNF703))
- Alternative Name
- ZNF703 (ZNF703 Products)
- Synonyms
- ZEPPO1 Protein, ZNF503L Protein, ZPO1 Protein, 1110032O19Rik Protein, AI430822 Protein, AL022941 Protein, Csmn1 Protein, End2 Protein, Zeppo1 Protein, Znf703 Protein, Zpo1 Protein, NH11 Protein, cb514 Protein, fc45d06 Protein, fk28e09 Protein, nlz Protein, nlz1 Protein, noz1 Protein, wu:fc45d06 Protein, wu:fk28e09 Protein, zgc:111927 Protein, nzl1 Protein, zinc finger protein 703 Protein, zinc finger protein 703 L homeolog Protein, zinc finger protein 703 S homeolog Protein, ZNF703 Protein, Zfp703 Protein, znf703 Protein, znf703.L Protein, znf703.S Protein
- Background
- Transcriptional corepressor which does not bind directly to DNA and may regulate transcription through recruitment of histone deacetylases to gene promoters. Regulates cell adhesion, migration and proliferation. May be required for segmental gene expression during hindbrain development. {ECO:0000269|PubMed:21328542, ECO:0000269|PubMed:21337521}.
- Molecular Weight
- 59.0 kDa Including tag.
- UniProt
- Q9H7S9
- Pathways
- Cell-Cell Junction Organization
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