PLEKHM3 Protein (AA 1-761) (Strep Tag)
Crystallography grade
PLEKHM3
Origin: Human
Host: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
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- Target See all PLEKHM3 products
- PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-761
- Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This PLEKHM3 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MEALEVDDIS PALEVTEEFF STLDSNLEKA VQQAEVYGIQ EVPELVGHEV LSNITDNGAM RNVTSLGKGG MIWDHCKSRL LETKAQNVFP AKEQFMVQRG TTPDNLSWME QKEASTFNFF NICQRRRDRP RSVNDLLDET STFKPGHARS RSDITQVDWR VVLKTTPLQQ QQQQQPLLQG PHVTRPSFLL PSPNKIEDAQ GNTEHKQTFP NILKKGYLEI RKDHDSYWQS CYAELSPYNL YFYSLDSSGN QNLYATYQLS HFQSISVLGN LEARMVDTVL YDNTQLQLKA ESPWEALDWG QKLWEVVHAA VPGYMGRQNE LTISPGLGHH DDYTQNHSFQ KKTSGLLPPS PVLDSSKQYQ NILKSGTLYR LTVQNNWKAF TFVLSRAYLM AFQPGKLDED PLLSYNVDVC LAVQMDNLDG CDSCFQVIFP QDVLRLRAET RQRAQEWMEA LKIAANVARS SEQNLQVTLR NKPKDQMGGH ELRKNKRQSV TTSFLSILTT LSLERGLTAQ SFKCAGCQRS IGLSNGKAKV CNYSGWYYCS SCHVDDSFLI PARIVHNWDT SKYKVSKQAK EFLEYVYEEP LIDIQQENAM LYHHAEPLAA VLRLRQRLKS LRAYLFSCRA AVAEDLRRRI FPREYLLQQI HLYSLADLQQ VIEGKLAPFL GKVIKFATSH VYSCSLCSQK GFICEICNNG EILYPFEDIS TSRCESCGAV FHSECKEKSV PCPRCVRREL QKKQKSFWQR LNMDESLEEA CTMFELSYQN T
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))
- Alternative Name
- PLEKHM3 (PLEKHM3 Products)
- Synonyms
- PLEKHM1L Protein, DAPR Protein, 9430067K14Rik Protein, A230102O09Rik Protein, AI449570 Protein, Plekhm1l Protein, Dapr Protein, RGD1307151 Protein, pleckstrin homology domain containing, family M, member 3 Protein, pleckstrin homology domain containing M3 Protein, pleckstrin homology domain containing M3 L homeolog Protein, PLEKHM3 Protein, Plekhm3 Protein, plekhm3.L Protein
- Background
- Pleckstrin homology domain-containing family M member 3 (PH domain-containing family M member 3) (Differentiation associated protein),FUNCTION: Involved in skeletal muscle differentiation. May act as a scaffold protein for AKT1 during muscle differentiation. {ECO:0000250|UniProtKB:Q8BM47}.
- Molecular Weight
- 87.2 kDa
- UniProt
- Q6ZWE6
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