UQCRC2 Protein (AA 15-453) (His tag)

Catalog No. ABIN3086785

Product Details

Target: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Protein Type: Recombinant

Protein Characteristics: AA 15-453

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UQCRC2 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: SLKVAPKVKA TAAPAGAPPQ PQDLEFTKLP NGLVIASLEN YSPVSRIGLF IKAGSRYEDF SNLGTTHLLR LTSSLTTKGA SSFKITRGIE AVGGKLSVTA TRENMAYTVE CLRGDVDILM EFLLNVTTAP EFRRWEVADL QPQLKIDKAV AFQNPQTHVI ENLHAAAYRN ALANPLYCPD YRIGKVTSEE LHYFVQNHFT SARMALIGLG VSHPVLKQVA EQFLNMRGGL GLSGAKANYR GGEIREQNGD SLVHAAFVAE SAVAGSAEAN AFSVLQHVLG AGPHVKRGSN TTSHLHQAVA KATQQPFDVS AFNASYSDSG LFGIYTISQA TAAGDVIKAA YNQVKTIAQG NLSNTDVQAA KNKLKAGYLM SVESSECFLE EVGSQALVAG SYMPPSTVLQ QIDSVANADI INAAKKFVSG QKSMAASGNL GHTPFVDEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human UQCRC2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Alternative Name: UQCRC2 (UQCRC2 Products)

Synonyms: MC3DN5 Protein, QCR2 Protein, UQCR2 Protein, fi06a11 Protein, uqcrc2 Protein, wu:fb64g04 Protein, wu:fi06a11 Protein, fb33d01 Protein, fc43c05 Protein, wu:fb33d01 Protein, wu:fc43c05 Protein, zgc:92453 Protein, MGC80228 Protein, MGC130698 Protein, Uqcrc2 Protein, Afu5g04210 Protein, AO090102000625 Protein, 1500004O06Rik Protein, UQCRC2 Protein, ubiquinol-cytochrome c reductase core protein 2 Protein, ubiquinol cytochrome c reductase core protein 2 Protein, ubiquinol-cytochrome c reductase core protein 2b Protein, ubiquinol-cytochrome c reductase core protein II Protein, ubiquinol-cytochrome c reductase core protein 2a Protein, ubiquinol-cytochrome c reductase core protein 2 L homeolog Protein, cytochrome b-c1 complex subunit 2, mitochondrial Protein, ubiquinol-cytochrome C reductase complex core protein 2 Protein, ubiquinol--cytochrome-c reductase subunit 2 Protein, UQCRC2 Protein, Uqcrc2 Protein, uqcrc2b Protein, uqcrc2a Protein, uqcrc2.L Protein, uqcrc2 Protein, LOC100026672 Protein, LOC100389097 Protein, LOC100456655 Protein, LOC100592631 Protein, AFUA_5G04210 Protein, ANI_1_872084 Protein, AOR_1_1048134 Protein, QCR2 Protein, LOC100861263 Protein

Background: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.

Molecular Weight: 47.7 kDa Including tag.

UniProt: P22695