KIAA1009 (KIAA1009) (AA 1-1403) protein (Strep Tag)

Catalog No. ABIN3091493

Product Details

Target: KIAA1009

Protein Type: Recombinant

Protein Characteristics: AA 1-1403

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MANCSQEELD EEFEQFMKEL SDDSFENSDK TARQSKKEMK KKDTVPWWIT EDDFKDDGLL GTNVSYLKTK KTSQPVMEIE EESAEKIQFL KSSGTSLLST DSLETNELVV SELNHSSLGV GLDTLEEQEE KEQFFARLEK GLTSSIDYSR LNKELDSNDS THFKALHSNQ ANAELTDDEH ENESKHEELA ENYSDDFEDE YVGAPLTTKD EEMPSKENSK SEKISVPKQE EEKTGMLANV VLLDSLDSVA EVNLDEQDKI TPKPRCLPEM TENEMTGTGV SYGQSSSDVE ALHQAYCHIA HSLGDEDKQK IESNTVEDIK SSVKGHPQEN EENSKNISTM ESDLPTVEEL MKPIRIDSFG ISGFDLQPVS SEKVAERKET EFFSSLPLKM NPNILSQDSQ HVNLFFDKND ENVILQKTTN ESMENSCPQV TEVTATEEHV DKMYLNILRK KITVNSSSLS QDDKINKTYR SQLSSEEEGA VMGKQVPYKK ARSAPPLLKR KPQSGLYASV RSSGYGKPSS PLKMFSTLEK KTSEDIIKSK NLRSISTSNQ PRKKEILSGT KLIKPAALDK PAHKTESCLS TRKKSENPTE TDSCIQFQTD SLGYCGENKE KKLLMFKRVQ EAEDKWRGAQ ALIEQIKATF SEKEKELENK LEELKKQQEK ELFKLNQDNY ILQAKLSSFE ETNKKQRWLH FGEAADPVTG EKLKQIQKEI QEQETLLQGY QQENERLYNQ VKDLQEQNKK NEERMFKENQ SLFSEVASLK EQMHKSRFLS QVVEDSEPTR NQNFTDLLAE LRMAQKEKDS LLEDIKRLKQ DKQALEVDFE KMKKERDQAK DQIAYVTGEK LYEIKILEET HKQEISRLQK RLQWYAENQE LLDKDALRLR EANEEIEKLK LEIEKLKAES GNPSIRQKIR LKDKAADAKK IQDLERQVKE MEGILKRRYP NSLPALILAA SAAGDTVDKN TVEFMEKRIK KLEADLEGKD EDAKKSLRTM EQQFQKMKIQ YEQRLEQQEQ LLACKLNQHD SPRIKALEKE LDDIKEAHQI TVRNLEAEID VLKHQNAELD VKKNDKDDED FQSIEFQVEQ AHAKAKLVRL NEELAAKKRE IQDLSKTVER LQKDRRMMLS NQNSKGREEM SAKRAKKDVL HSSKGNANSF PGTLDSKLYQ PHTFTDSHVS EVLQENYRLK NELEGLISEK NELKMKSEAV MNQFENSMRR VKEDTAAHIA SLKASHQREI EKLLCQNAVE NSSSKVAELN RKIATQEVLI RHFQSQVNEL QSKQESLVVS EVREEILQKE ITKLLEELRE AKENHTPEMK HFVGLEKKIK QMEMRHAQRE QELQQIIQQT HQVVETEQNK EVEKWKRLAQ LKNRELEKFR TELDSILDVL RELHRQGVVV PVAFADEMNA PEY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KIAA1009

Alternative Name: CEP162 (KIAA1009 Products)

Synonyms: C6orf84 Protein, CEP162 Protein, QN1 Protein, Cep162 Protein, mKIAA1009 Protein, Qn1 Protein, centrosomal protein 162 Protein, CEP162 Protein, Cep162 Protein

Background: Centrosomal protein of 162 kDa (Cep162) (Protein QN1 homolog),FUNCTION: Required to promote assembly of the transition zone in primary cilia. Acts by specifically recognizing and binding the axonemal microtubule. Localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base. Required to mediate CEP290 association with microtubules. {ECO:0000269|PubMed:23644468}.

Molecular Weight: 161.9 kDa

UniProt: Q5TB80