EIF3CL Protein (AA 1-914) (Strep Tag)

Catalog No. ABIN3092442

Product Details

Target: EIF3CL (Eukaryotic Translation Initiation Factor 3, Subunit C-Like (EIF3CL))

Protein Type: Recombinant

Protein Characteristics: AA 1-914

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This EIF3CL protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKAY GKAKSIVDKE GVPRFYIRIL ADLEDYLNEL WEDKEGKKKM NKNNAKALST LRQKIRKYNR DFESHITSYK QNPEQSADED AEKNEEDSEG SSDEDEDEDG VSAATFLKKK SEAPSGESRK FLKKMDDEDE DSEDSEDDED WDTGSTSSDS DSEEEEGKQT ALASRFLKKA PTTDEDKKAA EKKREDKAKK KHDRKSKRLD EEEEEDNEGG EWERVRGGVP LVKEKPKMFA KGTEITHAVV IKKLNEILQA RGKKGTDRAA QIELLQLLVQ IAAENNLGEG VIVKIKFNII ASLYDYNPNL ATYMKPEMWG KCLDCINELM DILFANPNIF VGENILEESE NLHNADQPLR VRGCILTLVE RMDEEFTKIM QNTDPHSQEY VEHLKDEAQV CAIIERVQRY LEEKGTTEEV CRIYLLRILH TYYKFDYKAH QRQLTPPEGS SKSEQDQAEN EGEDSAVLME RLCKYIYAKD RTDRIRTCAI LCHIYHHALH SRWYQARDLM LMSHLQDNIQ HADPPVQILY NRTMVQLGIC AFRQGLTKDA HNALLDIQSS GRAKELLGQG LLLRSLQERN QEQEKVERRR QVPFHLHINL ELLECVYLVS AMLLEIPYMA AHESDARRRM ISKQFHHQLR VGERQPLLGP PESMREHVVA ASKAMKMGDW KTCHSFIINE KMNGKVWDLF PEADKVRTML VRKIQEESLR TYLFTYSSVY DSISMETLSD MFELDLPTVH SIISKMIINE ELMASLDQPT QTVVMHRTEP TAQQNLALQL AEKLGSLVEN NERVFDHKQG TYGGYFRDQK DGYRKNEGYM RRGGYRQQQS QTAY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EIF3CL (Eukaryotic Translation Initiation Factor 3, Subunit C-Like (EIF3CL))

Alternative Name: EIF3CL (EIF3CL Products)

Synonyms: eukaryotic translation initiation factor 3 subunit C like Protein, EIF3CL Protein

Background: Eukaryotic translation initiation factor 3 subunit C-like protein,FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. {ECO:0000250|UniProtKB:Q99613}.

Molecular Weight: 105.5 kDa

UniProt: B5ME19