FAM135A Protein (AA 1-1515) (Strep Tag)

Catalog No. ABIN3092514

Product Details

Target: FAM135A (Family with Sequence Similarity 135, Member A (FAM135A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1515

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FAM135A protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MTEVQAMVEF SVELNKFYNV DLFQRGFYQI RASMKIPSRI PHRVEASLLH ATGMTLAFPA SVHDSLICSK TFQILYKNEE VVLNDVMIFK VKMLLDERKI EETLEEMNFL LSLDLHFTDG DYSADDLNAL QLISSRTLKL HFSPHRGLHH HVNVMFDYFH LSVVSVTVHA SLVALHQPLI SFPRPVKTTW LNRNAPAQNK DSVIPTLESV VFGINYTKQL SPDGCSFIIA DSFLHHAYRF HYTLCATLLL AFKGLHSYFI TVTEEIPSCQ KLELEEMDVE ARLTELCEEV KKIENPDELA ELINMNLAQL CSLLMALWGQ FLEVITLHEE LRILLAQEHH TLRVRRFSEA FFCFEHPREA AIAYQELHAQ SHLQMCTAIK NTSFCSSLPP LPIECSELDG DLNSLPIIFE DRYLDSVTED LDAPWMGIQN LQRSESSKMD KYETEESSVA GLSSPELKVR PAGASSIWYT EGEKQLTKSL KGKNEESNKS KVKVTKLMKT MKSENTKKLI KQNSKDSVVL VGYKCLKSTA SNDLIKCFEG NPSHSQKEGL DPTICGYNFD PKTYMRQTSQ KEASCLPTNT ERTEQKSPDI ENVQPDQFDP LNSGNLNLCA NLSISGKLDI SQDDSEITQM EHNLASRRSS DDCHDHQTTP SLGVRTIEIK PSNKDPFSGE NITVKLGPWT ELRQEEILVD NLLPNFESLE SNGKSKSIEI TFEKEALQEA KCLSIGESLT KLRSNLPAPS TKEYHVVVSG DTIKLPDISA TYASSRFSDS GVESEPSSFA THPNTDLVFE TVQGQGPCNS ERLFPQLLMK PDYNVKFSLG NHCTESTSAI SEIQSSLTSI NSLPSDDELS PDENSKKSVV PECHLNDSKT VLNLGTTDLP KCDDTKKSSI TLQQQSVVFS GNLDNETVAI HSLNSSIKDP LQFVFSDEET SSDVKSSCSS KPNLDTMCKG FQSPDKSNNS TGTAITLNSK LICLGTPCVI SGSISSNTDV SEDRTMKKNS DVLNLTQMYS EIPTVESETH LGTSDPFSAS TDIVKQGLVE NYFGSQSSTD ISDTCAVSYS NALSPQKETS EKEISNLQQE QDKEDEEEEQ DQQMVQNGYY EETDYSALDG TINAHYTSRD ELMEERLTKS EKINSDYLRD GINMPTVCTS GCLSFPSAPR ESPCNVKYSS KSKFDAITKQ PSSTSYNFTS SISWYESSPK PQIQAFLQAK EELKLLKLPG FMYSEVPLLA SSVPYFSVEE EDGSEDGVHL IVCVHGLDGN SADLRLVKTY IELGLPGGRI DFLMSERNQN DTFADFDSMT DRLLDEIIQY IQIYSLTVSK ISFIGHSLGN LIIRSVLTRP RFKYYLNKLH TFLSLSGPHL GTLYNSSALV NTGLWFMQKW KKSGSLLQLT CRDHSDPRQT FLYKLSNKAG LHYFKNVVLV GSLQDRYVPY HSARIEMCKT ALKDKQSGQI YSEMIHNLLR PVLQSKDCNL VRYNVINALP NTADSLIGRA AHIAVLDSEI FLEKFFLVAA LKYFQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FAM135A (Family with Sequence Similarity 135, Member A (FAM135A))

Alternative Name: FAM135A (FAM135A Products)

Synonyms: KIAA1411 Protein, 4921533L14Rik Protein, AI256732 Protein, AI835491 Protein, family with sequence similarity 135 member A Protein, family with sequence similarity 135, member A Protein, FAM135A Protein, Fam135a Protein

Background: Protein FAM135A

Molecular Weight: 169.8 kDa

UniProt: Q9P2D6