KIAA0556 Protein (KIAA0556) (AA 1-1618) (His tag)
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- Target See all KIAA0556 products
- KIAA0556
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1618
- Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This KIAA0556 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MDGQTLRKAE RSWSCSREKK EGYAKDMVTD FDEKHDEYLI LLQQRNRILK HLKSKDPVQL RLEHLEQGFS VYVNGANSEL KSSPRKAIHS DFSRSASHTE GTHDYGRRTL FREAEEALRR SSRTAPSKVQ RRGWHQKSVQ IRTEAGPRLH IEPPVDYSDD FELCGDVTLQ ANNTSEDRPQ ELRRSLELSV NLQRKQKDCS SDEYDSIEED ILSEPEPEDP ALVGHPRHDR PPSSGDWTQK DVHGEQETEG RSSPGPDTLV VLEFNPASKS HKRERNLSAK RKDNAEVFVP TKPEPNLTPQ APAVFPDQER MCSRPGSRRE RPLSATRKTL CEAEYPEEDA SAVLQAIQVE NAALQRALLS RKAEQPASPL QDAEGPPAKP WTSLLEEKEE TLELLPITTA TTTQEPAGAA GGARAINQAM DRIGLLGSRQ QQKLLKVLQA VESDSAHLGR VVSPTKEQVS DTEDKQRMRA DEIKDAIYVT MEILSNWGNS WWVGLTEVEF FDLNDTKLYV SPHDVDIRNT ATPGELGRLV NRNLAGKKDS SPWTCPFHPP LQLFFVIRNT RQLGDFHLAK IKVRNYWTAD GDLDIGAKNV KLYVNRNLIF NGKLDKGDRE APADHSILVD QKNEKSEQLE EAMNAHSEES KGTHEMAGAS GDKELGLGCS PPAETLADAK LSSQGNVSGK RKNSTNCRKD SLSQLEEYLR LSAVPTSMGD MPSAPATSPP VKCPPVHEEP SLIQQLENLM GRKICEPPGK TPSWLQPSPT GKDRKQGGRK PKPLWLSPEK PLAWKGRLPS DDVIGEGPGE TEARDKGLRH EPGWGTSRSV NTKERPQRAT TKVHSDDSDI FNQPPNRERP ASGRRGSRKD AGSSSHGDDQ PASREDTWSS RTPSRSRWRS EQEHTLHESW SSLSAFDRSH RGRISNTELP GDILDELLQQ KSSRHSDLPP SKKGEQPGLS RGQDGYSGET DAGGDFKIPV LPYGQRLVID IKSTWGDRHY VGLNGIEIFS SKGEPVQISN IKADPPDINI LPAYGKDPRV VTNLIDGVNR TQDDMHVWLA PFTRGRSHSI TIDFTHPCHV ALIRIWNYNK SRIHSFRGVK DITMLLDTQC IFEGEIAKAS GTLAGAPEHF GDTILFTTDD DILEAIFYSD EMFDLDVGSL DSLQDEEAMR RPSTADGEGD ERPFTQAGLG ADERIPELEL PSSSPVPQVT TPEPGIYHGI CLQLNFTASW GDLHYLGLTG LEVVGKEGQA LPIHLHQISA SPRDLNELPE YSDDSRALDK LIDGTNITME DEHMWLIPFS PGLDHVVTIR LDRAESIAGL RFWNYNKSPE DTYRGAKIVH VSLDGLCVSP PEGFLIRKGP GNCHFDFAQE ILFVDYLRAQ LLPQPARRLD MRSLECASMD YEAPLMPCGF IFQFQLLTSW GDPYYIGLTG LELYDERGEK IPLSENNIAA FPDSVNSLEG VGGDVRTPDK LIDQVNDTSD GRHMWLAPIL PGLVNRVYVI FDLPTTVSMI KLWNYAKTPH RGVKEFGLLV DDLLVYNGIL AMVSHLVGGI LPTCEPTVPY HTILFTEDRD IRHQEKHTTI SNQAEDQDVQ MMNENQIITN AKRKQSVVDP ALRPKTCISE KETRRRRC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human KIAA0556 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- KIAA0556
- Alternative Name
- KIAA0556 (KIAA0556 Products)
- Synonyms
- Kiaa0556 Protein, KIAA0556 Protein, RIKEN cDNA D430042O09 gene Protein, KIAA0556 Protein, D430042O09Rik Protein
- Background
- May influence the stability of microtubules (MT), possibly through interaction with the MT-severing katanin complex. {ECO:0000269|PubMed:26714646}.
- Molecular Weight
- 181.9 kDa Including tag.
- UniProt
- O60303
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