Laminin gamma 1 Protein (AA 34-1609) (His tag)

Catalog No. ABIN3093542

Product Details

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Protein Type: Recombinant

Protein Characteristics: AA 34-1609

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Laminin gamma 1 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: QAAMDECTDE GGRPQRCMPE FVNAAFNVTV VATNTCGTPP EEYCVQTGVT GVTKSCHLCD AGQPHLQHGA AFLTDYNNQA DTTWWQSQTM LAGVQYPSSI NLTLHLGKAF DITYVRLKFH TSRPESFAIY KRTREDGPWI PYQYYSGSCE NTYSKANRGF IRTGGDEQQA LCTDEFSDIS PLTGGNVAFS TLEGRPSAYN FDNSPVLQEW VTATDIRVTL NRLNTFGDEV FNDPKVLKSY YYAISDFAVG GRCKCNGHAS ECMKNEFDKL VCNCKHNTYG VDCEKCLPFF NDRPWRRATA ESASECLPCD CNGRSQECYF DPELYRSTGH GGHCTNCQDN TDGAHCERCR ENFFRLGNNE ACSSCHCSPV GSLSTQCDSY GRCSCKPGVM GDKCDRCQPG FHSLTEAGCR PCSCDPSGSI DECNIETGRC VCKDNVEGFN CERCKPGFFN LESSNPRGCT PCFCFGHSSV CTNAVGYSVY SISSTFQIDE DGWRAEQRDG SEASLEWSSE RQDIAVISDS YFPRYFIAPA KFLGKQVLSY GQNLSFSFRV DRRDTRLSAE DLVLEGAGLR VSVPLIAQGN SYPSETTVKY VFRLHEATDY PWRPALTPFE FQKLLNNLTS IKIRGTYSER SAGYLDDVTL ASARPGPGVP ATWVESCTCP VGYGGQFCEM CLSGYRRETP NLGPYSPCVL CACNGHSETC DPETGVCNCR DNTAGPHCEK CSDGYYGDST AGTSSDCQPC PCPGGSSCAV VPKTKEVVCT NCPTGTTGKR CELCDDGYFG DPLGRNGPVR LCRLCQCSDN IDPNAVGNCN RLTGECLKCI YNTAGFYCDR CKDGFFGNPL APNPADKCKA CNCNLYGTMK QQSSCNPVTG QCECLPHVTG QDCGACDPGF YNLQSGQGCE RCDCHALGST NGQCDIRTGQ CECQPGITGQ HCERCEVNHF GFGPEGCKPC DCHPEGSLSL QCKDDGRCEC REGFVGNRCD QCEENYFYNR SWPGCQECPA CYRLVKDKVA DHRVKLQELE SLIANLGTGD EMVTDQAFED RLKEAEREVM DLLREAQDVK DVDQNLMDRL QRVNNTLSSQ ISRLQNIRNT IEETGNLAEQ ARAHVENTER LIEIASRELE KAKVAAANVS VTQPESTGDP NNMTLLAEEA RKLAERHKQE ADDIVRVAKT ANDTSTEAYN LLLRTLAGEN QTAFEIEELN RKYEQAKNIS QDLEKQAARV HEEAKRAGDK AVEIYASVAQ LSPLDSETLE NEANNIKMEA ENLEQLIDQK LKDYEDLRED MRGKELEVKN LLEKGKTEQQ TADQLLARAD AAKALAEEAA KKGRDTLQEA NDILNNLKDF DRRVNDNKTA AEEALRKIPA INQTITEANE KTREAQQALG SAAADATEAK NKAHEAERIA SAVQKNATST KAEAERTFAE VTDLDNEVNN MLKQLQEAEK ELKRKQDDAD QDMMMAGMAS QAAQEAEINA RKAKNSVTSL LSIINDLLEQ LGQLDTVDLN KLNEIEGTLN KAKDEMKVSD LDRKVSDLEN EAKKQEAAIM DYNRDIEEIM KDIRNLEDIR KTLPSGCFNT PSIEKP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LAMC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Alternative Name: LAMC1 (LAMC1 Products)

Synonyms: lamb2 Protein, Lamb2 Protein, B2e Protein, sly Protein, LAMB2 Protein, putative laminin gamma-1 chain Protein, laminin subunit gamma 1 Protein, laminin, gamma 1 Protein, Smp_163810 Protein, lamc1 Protein, Lamc1 Protein, LAMC1 Protein

Background: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Molecular Weight: 175.2 kDa Including tag.

UniProt: P11047