NHSL1 Protein (AA 1-1610) (Strep Tag)

Catalog No. ABIN3094086

Product Details

Target: NHSL1 (NHS-Like 1 (NHSL1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1610

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NHSL1 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MKKEGSSGSF RLQPNTGSLS RAVSWINFSS LSRQTKRLFR SDGELSVCGQ QVEVDDENWI YRAQPRKAVS NLDEESRWTV HYTAPWHQQE NVFLPTTRPP CVEDLHRQAK LNLKSVLREC DKLRHDGYRS SQYYSQGPTF AANASPFCDD YQDEDEETDQ KCSLSSSEEE RFISIRRPKT PASSDFSDLN TQTNWTKSLP LPTPEEKMRQ QAQTVQADVV PINITASGTG QDDADGHSVY TPDHYSTLGR FNSCRSAGQR SETRDSSCQT EDVKVVPPSM RRIRAQKGQG IAAQMGHFSG SSGNMSVLSD SAGIVFPSRL DSDAGFHSLP RSGARANIQS LEPRLGALGP AGDMNGTFLY QRGHPQADEN LGHLGGASGT GTLLRPKSQE LRHFESENIM SPACVVSPHA TYSTSIIPNA TLSSSSEVIA IPTAQSAGQR ESKSSGSSHA RIKSRDHLIS RHAVKGDPQS PGRHWNEGHA TILSQDLDPH SPGEPALLSL CDSAVPLNAP ANRENGSQAM PYNCRNNLAF PAHPQDVDGK SESSYSGGGG HSSSEPWEYK SSGNGRASPL KPHLATPGYS TPTSNMSSCS LDQTSNKEDA GSLYSEDHDG YCASVHTDSG HGSGNLCNSS DGFGNPRHSV INVFVGRAQK NQGDRSNYQD KSLSRNISLK KAKKPPLPPS RTDSLRRIPK KSSQCNGQVL NESLIATLQH SLQLSLPGKS GSSPSQSPCS DLEEPWLPRS RSQSTVSAGS SMTSATTPNV YSLCGATPSQ SDTSSVKSEY TDPWGYYIDY TGMQEDPGNP AGGCSTSSGV PTGNGPVRHV QEGSRATMPQ VPGGSVKPKI MSPEKSHRVI SPSSGYSSQS NTPTALTPVP VFLKSVSPAN GKGKPKPKVP ERKSSLISSV SISSSSTSLS SSTSTEGSGT MKKLDPAVGS PPAPPPPPVP SPPFPCPADR SPFLPPPPPV TDCSQGSPLP HSPVFPPPPP EALIPFCSPP DWCLSPPRPA LSPILPDSPV SLPLPPPLLP SSEPPPAPPL DPKFMKDTRP PFTNSGQPES SRGSLRPPST KEETSRPPMP LITTEALQMV QLRPVRKNSG AEAAQLSERT AQEQRTPVAP QYHLKPSAFL KSRNSTNEME SESQPASVTS SLPTPAKSSS QGDHGSAAER GGPVSRSPGA PSAGEAEARP SPSTTPLPDS SPSRKPPPIS KKPKLFLVVP PPQKDFAVEP AENVSEALRA VPSPTTGEEG SVHSREAKES SAAQAGSHAT HPGTSVLEGG AAGSMSPSRV EANVPMVQPD VSPAPKQEEP AENSADTGGD GESCLSQQDG AAGVPETNAA GSSSEACDFL KEDGNDEVMT PSRPRTTEDL FAAIHRSKRK VLGRRDSDDD HSRNHSPSPP VTPTGAAPSL ASPKQVGSIQ RSIRKSSTSS DNFKALLLKK GSRSDTSARM SAAEMLKNTD PRFQRSRSEP SPDAPESPSS CSPSKNRRAQ EEWAKNEGLM PRSLSFSGPR YGRSRTPPSA ASSRYSMRNR IQSSPMTVIS EGEGEAVEPV DSIARGALGA AEGCSLDGLA REEMDEGGLL CGEGPAASLQ PQAPGPVDGT ASAEGREPSP QCGGSLSEES
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NHSL1 (NHS-Like 1 (NHSL1))

Alternative Name: NHSL1 (NHSL1 Products)

Synonyms: gukh2 Protein, nhsl1 Protein, si:ch211-195e3.6 Protein, NHS Protein, C6orf63 Protein, 5730409E15Rik Protein, A630035H13Rik Protein, A730096F01 Protein, BC013565 Protein, D10Bwg0940e Protein, mKIAA1357 Protein, NHS like 1 Protein, NHS-like 1b Protein, NHS-like 1 Protein, NHSL1 Protein, nhsl1b Protein, nhsl1 Protein, LOC100222190 Protein, Nhsl1 Protein

Background: NHS-like protein 1

Molecular Weight: 170.7 kDa

UniProt: Q5SYE7