RBP3 Protein (AA 18-1247) (His tag)

Catalog No. ABIN3094986

Product Details

Target: RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))

Protein Type: Recombinant

Protein Characteristics: AA 18-1247

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This RBP3 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: GPTHLFQPSL VLDMAKVLLD NYCFPENLLG MQEAIQQAIK SHEILSISDP QTLASVLTAG VQSSLNDPRL VISYEPSTPE PPPQVPALTS LSEEELLAWL QRGLRHEVLE GNVGYLRVDS VPGQEVLSMM GEFLVAHVWG NLMGTSALVL DLRHCTGGQV SGIPYIISYL HPGNTILHVD TIYNRPSNTT TEIWTLPQVL GERYGADKDV VVLTSSQTRG VAEDIAHILK QMRRAIVVGE RTGGGALDLR KLRIGESDFF FTVPVSRSLG PLGGGSQTWE GSGVLPCVGT PAEQALEKAL AILTLRSALP GVVHCLQEVL KDYYTLVDRV PTLLQHLASM DFSTVVSEED LVTKLNAGLQ AASEDPRLLV RAIGPTETPS WPAPDAAAED SPGVAPELPE DEAIRQALVD SVFQVSVLPG NVGYLRFDSF ADASVLGVLA PYVLRQVWEP LQDTEHLIMD LRHNPGGPSS AVPLLLSYFQ GPEAGPVHLF TTYDRRTNIT QEHFSHMELP GPRYSTQRGV YLLTSHRTAT AAEEFAFLMQ SLGWATLVGE ITAGNLLHTR TVPLLDTPEG SLALTVPVLT FIDNHGEAWL GGGVVPDAIV LAEEALDKAQ EVLEFHQSLG ALVEGTGHLL EAHYARPEVV GQTSALLRAK LAQGAYRTAV DLESLASQLT ADLQEVSGDH RLLVFHSPGE LVVEEAPPPP PAVPSPEELT YLIEALFKTE VLPGQLGYLR FDAMAELETV KAVGPQLVRL VWQQLVDTAA LVIDLRYNPG SYSTAIPLLC SYFFEAEPRQ HLYSVFDRAT SKVTEVWTLP QVAGQRYGSH KDLYILMSHT SGSAAEAFAH TMQDLQRATV IGEPTAGGAL SVGIYQVGSS PLYASMPTQM AMSATTGKAW DLAGVEPDIT VPMSEALSIA QDIVALRAKV PTVLQTAGKL VADNYASAEL GAKMATKLSG LQSRYSRVTS EVALAEILGA DLQMLSGDPH LKAAHIPENA KDRIPGIVPM QIPSPEVFEE LIKFSFHTNV LEDNIGYLRF DMFGDGELLT QVSRLLVEHI WKKIMHTDAM IIDMRFNIGG PTSSIPILCS YFFDEGPPVL LDKIYSRPDD SVSELWTHAQ VVGERYGSKK SMVILTSSVT AGTAEEFTYI MKRLGRALVI GEVTSGGCQP PQTYHVDDTN LYLTIPTARS VGASDGSSWE GVGVTPHVVV PAEEALARAK EMLQHNQLRV KRSPGLQDHL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RBP3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))

Alternative Name: RBP3 (RBP3 Products)

Synonyms: Irbp Protein, Rbp-3 Protein, D10S64 Protein, D10S65 Protein, D10S66 Protein, IRBP Protein, RBPI Protein, RP66 Protein, retinol binding protein 3 Protein, interphotoreceptor retinoid-binding protein Protein, retinol binding protein 3, interstitial Protein, rbp3 Protein, RBP3 Protein, glr2547 Protein, XOO2681 Protein, AMED_0162 Protein, ambt_08395 Protein, Rbp3 Protein

Background: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.

Molecular Weight: 134.4 kDa Including tag.

UniProt: P10745