SALL3 Protein (AA 1-1300) (Strep Tag)

Catalog No. ABIN3117813

Product Details

Target: SALL3 (Sal-Like 3 (SALL3))

Protein Type: Recombinant

Protein Characteristics: AA 1-1300

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SALL3 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSRRKQAKPQ HLKSDEELLP PDGAPEHAAP GEGAEDADSG PESRSGGEET SVCEKCCAEF FKWADFLEHQ RSCTKLPPVL IVHEDAPAPP PEDFPEPSPA SSPSERAESE AAEEAGAEGA EGEARPVEKE AEPMDAEPAG DTRAPRPPPA APAPPTPAYG APSTNVTLEA LLSTKVAVAQ FSQGARAAGG SGAGGGVAAA AVPLILEQLM ALQQQQIHQL QLIEQIRSQV ALMQRPPPRP SLSPAAAPSA PGPAPSQLPG LAALPLSAGA PAAAIAGSGP AAPAAFEGAQ PLSRPESGAS TPGGPAEPSA PAAPSAAPAP AAPAPAPAPQ SAASSQPQSA STPPALAPGS LLGAAPGLPS PLLPQTSASG VIFPNPLVSI AATANALDPL SALMKHRKGK PPNVSVFEPK ASAEDPFFKH KCRFCAKVFG SDSALQIHLR SHTGERPFKC NICGNRFSTK GNLKVHFQRH KEKYPHIQMN PYPVPEYLDN VPTCSGIPYG MSLPPEKPVT TWLDSKPVLP TVPTSVGLQL PPTVPGAHGY ADSPSATPAS RSPQRPSPAS SECASLSPGL NHVESGVSAT AESPQSLLGG PPLTKAEPVS LPCTNARAGD APVGAQASAA PTSVDGAPTS LGSPGLPAVS EQFKAQFPFG GLLDSMQTSE TSKLQQLVEN IDKKMTDPNQ CVICHRVLSC QSALKMHYRT HTGERPFKCK ICGRAFTTKG NLKTHFGVHR AKPPLRVQHS CPICQKKFTN AVVLQQHIRM HMGGQIPNTP LPEGFQDAMD SELAYDDKNA ETLSSYDDDM DENSMEDDAE LKDAATDPAK PLLSYAGSCP PSPPSVISSI AALENQMKMI DSVMSCQQLT GLKSVENGSG ESDRLSNDSS SAVGDLESRS AGSPALSESS SSQALSPAPS NGESFRSKSP GLGAPEEPQE IPLKTERPDS PAAAPGSGGA PGRAGIKEEA PFSLLFLSRE RGKCPSTVCG VCGKPFACKS ALEIHYRSHT KERPFVCALC RRGCSTMGNL KQHLLTHRLK ELPSQLFDPN FALGPSQSTP SLISSAAPTM IKMEVNGHGK AMALGEGPPL PAGVQVPAGP QTVMGPGLAP MLAPPPRRTP KQHNCQSCGK TFSSASALQI HERTHTGEKP FGCTICGRAF TTKGNLKVHM GTHMWNNAPA RRGRRLSVEN PMALLGGDAL KFSEMFQKDL AARAMNVDPS FWNQYAAAIT NGLAMKNNEI SVIQNGGIPQ LPVSLGGSAL PPLGSMASGM DKARTGSSPP IVSLDKASSE TAASRPFTRF IEDNKEIGIN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SALL3 (Sal-Like 3 (SALL3))

Alternative Name: SALL3 (SALL3 Products)

Synonyms: ZNF796 Protein, B130022O04Rik Protein, Msal Protein, Msal-1 Protein, Sal Protein, Salt Protein, Spalt Protein, Xsal-1 Protein, Xsal-3 Protein, sal1-A Protein, SAL2 Protein, znf796 Protein, sall3 Protein, spa3 Protein, spalt like transcription factor 3 Protein, sal-like 3 (Drosophila) Protein, spalt-like transcription factor 3 Protein, spalt-like transcription factor 3 L homeolog Protein, spalt-like transcription factor 3 S homeolog Protein, spalt-like transcription factor 3a Protein, SALL3 Protein, Sall3 Protein, sall3.L Protein, sall3.S Protein, sall3 Protein, sall3a Protein

Background: Sal-like protein 3 (Zinc finger protein 796) (Zinc finger protein SALL3) (hSALL3),FUNCTION: Probable transcription factor.

Molecular Weight: 135.3 kDa

UniProt: Q9BXA9