TDRD9 Protein (AA 1-1382) (Strep Tag)

Catalog No. ABIN3095867

Product Details

Target: TDRD9 (Tudor Domain Containing 9 (TDRD9))

Protein Type: Recombinant

Protein Characteristics: AA 1-1382

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TDRD9 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MLRKLTIEQI NDWFTIGKTV TNVELLGAPP AFPAGAAREE VQRQDVAPGA GPAAQAPALA QAPARPAAAF ERSLSQRSSE VEYINKYRQL EAQELDVCRS VQPTSGPGPR PSLAKLSSVT CIPGTTYKYP DLPISRYKEE VVSLIESNSV VIIHGATGSG KSTQLPQYIL DHYVQRSAYC SIVVTQPRKI GASSIARWIS KERAWTLGGV VGYQVGLEKI ATEDTRLIYM TTGVLLQKIV SAKSLMEFTH IIIDEVHERT EEMDFLLLVV RKLLRTNSRF VKVVLMSATI SCKEFADYFA VPVQNKMNPA YIFEVEGKPH SVEEYYLNDL EHIHHSKLSP HLLEEPVITK DIYEVAVSLI QMFDDLDMKE SGNKAWSGAQ FVLERSSVLV FLPGLGEINY MHELLTSLVH KRLQVYPLHS SVALEEQNNV FLSPVPGYRK IILSTNIAES SVTVPDVKYV IDFCLTRTLV CDEDTNYQSL RLSWASKTSC NQRKGRAGRV SRGYCYRLVH KDFWDNSIPD HVVPEMLRCP LGSTILKVKL LDMGEPRALL ATALSPPGLS DIERTILLLK EVGALAVSGQ REDENPHDGE LTFLGRVLAQ LPVNQQLGKL IVLGHVFGCL DECLIIAAAL SLKNFFAMPF RQHLDGYRNK VNFSGSSKSD CIALVEAFKT WKACRQTGEL RYPKDELNWG RLNYIQIKRI REVAELYEEL KTRISQFNMH VDSRRPVMDQ EYIYKQRFIL QVVLAGAFYP NYFTFGQPDE EMAVRELAGK DPKTTVVLKH IPPYGFLYYK QLQSLFRQCG QVKSIVFDGA KAFVEFSRNP TERFKTLPAV YMAIKMSQLK VSLELSVHSA EEIEGKVQGM NVSKLRNTRV NVDFQKQTVD PMQVSFNTSD RSQTVTDLLL TIDVTEVVEV GHFWGYRIDE NNSEILKKLT AEINQLTLVP LPTHPHPDLV CLAPFADFDK QRYFRAQVLY VSGNSAEVFF VDYGNKSHVD LHLLMEIPCQ FLELPFQALE FKICKMRPSA KSLVCGKHWS DGASQWFASL VSGCTLLVKV FSVVHSVLHV DVYQYSGVQD AINIRDVLIQ QGYAELTEES YESKQSHEVL KGLFSKSVEN MTDGSVPFPM KDDEKYLIRI LLESFSTNKL GTPNCKAELH GPFNPYELKC HSLTRISKFR CVWIEKESIN SVIISDAPED LHQRMLVAAS LSINATGSTM LLRETSLMPH IPGLPALLSM LFAPVIELRI DQNGKYYTGV LCGLGWNPAT GASILPEHDM ELAFDVQFSV EDVVEVNILR AAINKLVCDG PNGCKCLGPE RVAQLQDIAR QKLLGLFCQS KPREKIVPKW HEKPYEWNQV DPKLVMEQAD RESSRGKNTF LYQLHKLVVL GT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TDRD9 (Tudor Domain Containing 9 (TDRD9))

Alternative Name: TDRD9 (TDRD9 Products)

Synonyms: MGC146806 Protein, C14orf75 Protein, HIG-1 Protein, NET54 Protein, 4930441E05Rik Protein, si:dkey-15f17.9 Protein, tdrd9l Protein, tudor domain containing 9 Protein, TDRD9 Protein, tdrd9 Protein, Tdrd9 Protein

Background: ATP-dependent RNA helicase TDRD9 (EC 3.6.4.13) (Tudor domain-containing protein 9),FUNCTION: ATP-binding RNA helicase required during spermatogenesis (PubMed:28536242). Required to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Acts downstream of piRNA biogenesis: exclusively required for transposon silencing in the nucleus, suggesting that it acts as a nuclear effector in the nucleus together with PIWIL4. {ECO:0000250|UniProtKB:Q14BI7, ECO:0000269|PubMed:28536242}.

Molecular Weight: 155.7 kDa

UniProt: Q8NDG6