TTC37 Protein (AA 2-1564) (His tag)
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- Target See all TTC37 products
- TTC37 (Tetratricopeptide Repeat Domain 37 (TTC37))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-1564
- Origin
- Human
- Source
- Insect Cells
- Purification tag / Conjugate
- This TTC37 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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SSKEVKTALK SARDAIRNKE YKEALKHCKT VLKQEKNNYN AWVFIGVAAA ELEQPDQAQS AYKKAAELEP DQLLAWQGLA NLYEKYNHIN AKDDLPGVYQ KLLDLYESVD KQKWCDVCKK LVDLYYQEKK HLEVARTWHK LIKTRQEQGA ENEELHQLWR KLTQFLAEST EDQNNETQQL LFTAFENALG LSDKIPSEDH QVLYRHFIQS LSKFPHESAR LKKACEGMIN IYPTVQYPLE VLCLHLIESG NLTDEGQQYC CRLVEMDSKS GPGLIGLGIK ALQDKKYEDA VRNLTEGLKE SPVCTSGWYH LAEAQVKMHR PKEAVLSCSQ ALKIVDNLGA SGNSLYQRNL CLHLKAEALI KLSDYDSSEE AIRTLDQISD ADNIPGLLVL KSLAYRNKGS FDEAAKIMED LLSSYPDLAE VHALEALIHF TKKDYLQAEK CFQRALEKDT EVAEYHYQLG LTYWFMGEET RKDKTKALTH FLKAARLDTY MGKVFCYLGH YYRDVVGDKN RARGCYRKAF ELDDTDAESG AAAVDLSVEL EDMEMALAIL TTVTQKASAG TAKWAWLRRG LYYLKAGQHS QAVADLQAAL RADPKDFNCW ESLGEAYLSR GGYTTALKSF TKASELNPES IYSVFKVAAI QQILGKYKEA VAQYQMIIKK KEDYVPALKG LGECHLMMAK AALVDYLDGK AVDYIEKALE YFTCALQHRA DVSCLWKLAG DACTCLYAVA PSKVNVHVLG VLLGQKEGKQ VLKKNELLHL GGRCYGRALK LMSTSNTWCD LGINYYRQAQ HLAETGSNMN DLKELLEKSL HCLKKAVRLD SNNHLYWNAL GVVACYSGIG NYALAQHCFI KSIQSEQINA VAWTNLGVLY LTNENIEQAH EAFKMAQSLD PSYLMCWIGQ ALIAEAVGSY DTMDLFRHTT ELNMHTEGAL GYAYWVCTTL QDKSNRETEL YQYNILQMNA IPAAQVILNK YVERIQNYAP AFTMLGYLNE HLQLKKEAAN AYQRAILLLQ TAEDQDTYNV AIRNYGRLLC STGEYDKAIQ AFKSTPLEVL EDIIGFALAL FMKGLYKESS KAYERALSIV ESEQDKAHIL TALAITEYKQ GKTDVAKTLL FKCSILKEPT TESLQALCAL GLAMQDATLS KAALNELLKH IKHKDSNYQR CLLTSAIYAL QGRSVAVQKQ ISKAVHSNPG DPALWSLLSR VVAQYAQRNA KGGVVAGNVA HILDSNHGKK ALLYTAVNQL AMGSSSAEDE KNTALKTIQK AALLSPGDPA IWAGLMAACH ADDKLALVNN TQPKRIDLYL ALLSAVSASI KDEKFFENYN QSLEKWSLSQ AVTGLIDTGR ISEAETLCTK NLKSNPDQPA VILLLRQVQC KPLLESQKPL PDAVLEELQK TVMSNSTSVP AWQWLAHVYQ SQGMMRAAEM CYRKSLQLAS QRGSWSGKLS SLLRLALLAL KVCMANISND HWPSLVQEAT TEALKLCFCP LAVLLQALLQ FKRKMGARET RRLLERVVYQ PGYPKSIAST ARWYLLRHLY AKDDYELIDV LVNNAKTHGD TRALELNQRL SSQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TTC37 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- TTC37 (Tetratricopeptide Repeat Domain 37 (TTC37))
- Alternative Name
- TTC37 (TTC37 Products)
- Synonyms
- KIAA0372 Protein, Ski3 Protein, tetratricopeptide repeat domain 37 Protein, tetratricopeptide repeat domain 37 L homeolog Protein, TTC37 Protein, ttc37.L Protein
- Background
- Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C).
- Molecular Weight
- 176.3 kDa Including tag.
- UniProt
- Q6PGP7
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