USP31 Protein (AA 1-1352) (Strep Tag)

Catalog No. ABIN3096233

Product Details

Target: USP31 (Ubiquitin Specific Peptidase 31 (USP31))

Protein Type: Recombinant

Protein Characteristics: AA 1-1352

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This USP31 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSKVTAPGSG PPAAASGKEK RSFSKRLFRS GRAGGGGAGG PGASGPAAPS SPSSPSSARS VGSFMSRVLK TLSTLSHLSS EGAAPDRGGL RSCFPPGPAA APTPPPCPPP PASPAPPACA AEPVPGVAGL RNHGNTCFMN ATLQCLSNTE LFAEYLALGQ YRAGRPEPSP DPEQPAGRGA QGQGEVTEQL AHLVRALWTL EYTPQHSRDF KTIVSKNALQ YRGNSQHDAQ EFLLWLLDRV HEDLNHSVKQ SGQPPLKPPS ETDMMPEGPS FPVCSTFVQE LFQAQYRSSL TCPHCQKQSN TFDPFLCISL PIPLPHTRPL YVTVVYQGKC SHCMRIGVAV PLSGTVARLR EAVSMETKIP TDQIVLTEMY YDGFHRSFCD TDDLETVHES DCIFAFETPE IFRPEGILSQ RGIHLNNNLN HLKFGLDYHR LSSPTQTAAK QGKMDSPTSR AGSDKIVLLV CNRACTGQQG KRFGLPFVLH LEKTIAWDLL QKEILEKMKY FLRPTVCIQV CPFSLRVVSV VGITYLLPQE EQPLCHPIVE RALKSCGPGG TAHVKLVVEW DKETRDFLFV NTEDEYIPDA ESVRLQRERH HQPQTCTLSQ CFQLYTKEER LAPDDAWRCP HCKQLQQGSI TLSLWTLPDV LIIHLKRFRQ EGDRRMKLQN MVKFPLTGLD MTPHVVKRSQ SSWSLPSHWS PWRRPYGLGR DPEDYIYDLY AVCNHHGTMQ GGHYTAYCKN SVDGLWYCFD DSDVQQLSED EVCTQTAYIL FYQRRTAIPS WSANSSVAGS TSSSLCEHWV SRLPGSKPAS VTSAASSRRT SLASLSESVE MTGERSEDDG GFSTRPFVRS VQRQSLSSRS SVTSPLAVNE NCMRPSWSLS AKLQMRSNSP SRFSGDSPIH SSASTLEKIG EAADDKVSIS CFGSLRNLSS SYQEPSDSHS RREHKAVGRA PLAVMEGVFK DESDTRRLNS SVVDTQSKHS AQGDRLPPLS GPFDNNNQIA YVDQSDSVDS SPVKEVKAPS HPGSLAKKPE STTKRSPSSK GTSEPEKSLR KGRPALASQE SSLSSTSPSS PLPVKVSLKP SRSRSKADSS SRGSGRHSSP APAQPKKESS PKSQDSVSSP SPQKQKSASA LTYTASSTSA KKASGPATRS PFPPGKSRTS DHSLSREGSR QSLGSDRASA TSTSKPNSPR VSQARAGEGR GAGKHVRSSS MASLRSPSTS IKSGLKRDSK SEDKGLSFFK SALRQKETRR STDLGKTALL SKKAGGSSVK SVCKNTGDDE AERGHQPPAS QQPNANTTGK EQLVTKDPAS AKHSLLSARK SKSSQLDSGV PSSPGGRQSA EKSSKKLSSS MQTSARPSQK PQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: USP31 (Ubiquitin Specific Peptidase 31 (USP31))

Alternative Name: USP31 (USP31 Products)

Synonyms: USP31 Protein, USP48 Protein, mKIAA1203 Protein, 6330567E21Rik Protein, ubiquitin specific peptidase 31 Protein, USP31 Protein, usp31 Protein, Usp31 Protein

Background: Ubiquitin carboxyl-terminal hydrolase 31 (EC 3.4.19.12) (Deubiquitinating enzyme 31) (Ubiquitin thioesterase 31) (Ubiquitin-specific-processing protease 31),FUNCTION: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity). {ECO:0000250}.

Molecular Weight: 146.7 kDa

UniProt: Q70CQ4