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ZNF609 Protein (AA 1-1411) (Strep Tag)

Crystallography grade ZNF609 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Catalog No. ABIN3096466
  • Target See all ZNF609 products
    ZNF609 (Zinc Finger Protein 609 (ZNF609))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1411
    Origin
    Human
    Source
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This ZNF609 protein is labelled with Strep Tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MSLSSGASGG KGVDANPVET YDSGDEWDIG VGNLIIDLDA DLEKDQQKLE MSGSKEVGIP APNAVATLPD NIKFVTPVPG PQGKEGKSKS KRSKSGKDTS KPTPGTSLFT PSEGAASKKE VQGRSGDGAN AGGLVAAIAP KGSEKAAKAS RSVAGSKKEK ENSSSKSKKE RSEGVGTCSE KDPGVLQPVP LGGRGGQYDG SAGVDTGAVE PLGSIAIEPG AALNPLGTKP EPEEGENECR LLKKVKSEKM ESPVSTPAVL PIHLLVPVVN NDISSPCEQI MVRTRSVGVN TCDVALATEP ECLGPCEPGT SVNLEGIVWQ ETEDGMLVVN VTWRNKTYVG TLLDCTRHDW APPRFCDSPT SDLEMRNGRG RGKRMRPNSN TPVNETATAS DSKGTSNSSK TRAGANSKGR RGSQNSSEHR PPASSTSEDV KASPSSANKR KNKPLSDMEL NSSSEDSKGS KRVRTNSMGS ATGPLPGTKV EPTVLDRNCP SPVLIDCPHP NCNKKYKHIN GLKYHQAHAH TDDDSKPEAD GDSEYGEEPI LHADLGSCNG ASVSQKGSLS PARSATPKVR LVEPHSPSPS SKFSTKGLCK KKLSGEGDTD LGALSNDGSD DGPSVMDETS NDAFDSLERK CMEKEKCKKP SSLKPEKIPS KSLKSARPIA PAIPPQQIYT FQTATFTAAS PGSSSGLTAT VAQAMPNSPQ LKPIQPKPTV MGEPFTVNPA LTPAKDKKKK DKKKKESSKE LESPLTPGKV CRAEEGKSPF RESSGDGMKM EGLLNGSSDP HQSRLASIKA EADKIYSFTD NAPSPSIGGS SRLENTTPTQ PLTPLHVVTQ NGAEASSVKT NSPAYSDISD AGEDGEGKVD SVKSKDAEQL VKEGAKKTLF PPQPQSKDSP YYQGFESYYS PSYAQSSPGA LNPSSQAGVE SQALKTKRDE EPESIEGKVK NDICEEKKPE LSSSSQQPSV IQQRPNMYMQ SLYYNQYAYV PPYGYSDQSY HTHLLSTNTA YRQQYEEQQK RQSLEQQQRG VDKKAEMGLK EREAALKEEW KQKPSIPPTL TKAPSLTDLV KSGPGKAKEP GADPAKSVII PKLDDSSKLP GQAPEGLKVK LSDASHLSKE ASEAKTGAEC GRQAEMDPIL WYRQEAEPRM WTYVYPAKYS DIKSEDERWK EERDRKLKEE RSRSKDSVPK EDGKESTSSD CKLPTSEESR LGSKEPRPSV HVPVSSPLTQ HQSYIPYMHG YSYSQSYDPN HPSYRSMPAV MMQNYPGSYL PSSYSFSPYG SKVSGGEDAD KARASPSVTC KSSSESKALD ILQQHASHYK SKSPTISDKT SQERDRGGCG VVGGGGSCSS VGGASGGERS VDRPRTSPSQ RLMSTHHHHH HLGYSLLPAQ YNLPYAAGLS STAIVASQQG STPSLYPPPR R
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ZNF609 (Zinc Finger Protein 609 (ZNF609))
    Alternative Name
    ZNF609 (ZNF609 Products)
    Synonyms
    ZNF609 Protein, RGD1561651 Protein, Znf609 Protein, 9830165N24 Protein, BC016271 Protein, mKIAA0295 Protein, fi39d03 Protein, wu:fi39d03 Protein, zgc:162740 Protein, zinc finger protein 609 Protein, zinc finger protein 609a Protein, ZNF609 Protein, znf609 Protein, Zfp609 Protein, Znf609 Protein, znf609a Protein
    Background
    Zinc finger protein 609,FUNCTION: Transcription factor, which activates RAG1, and possibly RAG2, transcription. Through the regulation of RAG1/2 expression, may regulate thymocyte maturation. Along with NIPBL and the multiprotein complex Integrator, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others. {ECO:0000250|UniProtKB:Q8BZ47}., FUNCTION: [Isoform 2]: Involved in the regulation of myoblast proliferation during myogenesis. {ECO:0000269|PubMed:28344082}.
    Molecular Weight
    151.2 kDa
    UniProt
    O15014
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