ZBTB38 Protein (AA 1-1195) (Strep Tag)

Catalog No. ABIN3096475

Product Details

Target: ZBTB38 (Zinc Finger and BTB Domain Containing 38 (ZBTB38))

Protein Type: Recombinant

Protein Characteristics: AA 1-1195

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ZBTB38 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MTVMSLSRDL KDDFHSDTVL SILNEQRIRG ILCDVTIIVE DTKFKAHSNV LAASSLYFKN IFWSHTICIS SHVLELDDLK AEVFTEILNY IYSSTVVVKR QETVTDLAAA GKKLGISFLE DLTDRNFSNS PGPYVFCITE KGVVKEEKNE KRHEEPAITN GPRITNAFSI IETENSNNMF SPLDLRASFK KVSDSMRTAS LCLERTDVCH EAEPVRTLAE HSYAVSSVAE AYRSQPVREH DGSSPGNTGK ENCEALAAKP KTCRKPKTFS IPQDSDSATE NIPPPPVSNL EVNQERSPQP AAVLTRSKSP NNEGDVHFSR EDENQSSDVP GPPAAEVPPL VYNCSCCSKA FDSSTLLSAH MQLHKPTQEP LVCKYCNKQF TTLNRLDRHE QICMRSSHMP IPGGNQRFLE NYPTIGQNGG SFTGPEPLLS ENRIGEFSST GSTLPDTDHM VKFVNGQMLY SCVVCKRSYV TLSSLRRHAN VHSWRRTYPC HYCNKVFALA EYRTRHEIWH TGERRYQCIF CLETFMTYYI LKNHQKSFHA IDHRLSISKK TANGGLKPSV YPYKLYRLLP MKCKRAPYKS YRNSSYENAR ENSQMNESAP GTYVVQNPHS SELPTLNFQD TVNTLTNSPA IPLETSACQD IPTSANVQNA EGTKWGEEAL KMDLDNNFYS TEVSVSSTEN AVSSDLRAGD VPVLSLSNSS ENAASVISYS GSAPSVIVHS SQFSSVIMHS NAIAAMTSSN HRAFSDPAVS QSLKDDSKPE PDKVGRFASR PKSIKEKKKT TSHTRGEIPE ESNYVADPGG SLSKTTNIAE ETSKIETYIA KPALPGTSTN SNVAPLCQIT VKIGNEAIVK RHILGSKLFY KRGRRPKYQM QEEPLPQGND PEPSGDSPLG LCQSECMEMS EVFDDASDQD STDKPWRPYY NYKPKKKSRQ LKKMRKVNWR KEHGNRSPSH KCKYPAELDC AVGKAPQDKP FEEEETKEMP KLQCELCDGD KAVGAGNQGR PHRHLTSRPY ACELCAKQFQ SPSTLKMHMR CHTGEKPYQC KTCGRCFSVQ GNLQKHERIH LGLKEFVCQY CNKAFTLNET LKIHERIHTG EKRYHCQFCF QRFLYLSTKR NHEQRHIREH NGKGYACFQC PKICKTAAAL GMHQKKHLFK SPSQQEKIGD VCHENSNPLE NQHFIGSEDN DQKDNIQTGV ENVVL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ZBTB38 (Zinc Finger and BTB Domain Containing 38 (ZBTB38))

Alternative Name: ZBTB38 (ZBTB38 Products)

Synonyms: ZBTB38 Protein, CIBZ Protein, ZNF921 Protein, A930014K01Rik Protein, RGD1310136 Protein, zinc finger and BTB domain containing 38 Protein, ZBTB38 Protein, Zbtb38 Protein

Background: Zinc finger and BTB domain-containing protein 38,FUNCTION: Transcriptional regulator with bimodal DNA-binding specificity. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to E-box elements (5'-CACGTG-3'). Can also bind specifically to a single methyl-CpG pair. Represses transcription in a methyl-CpG-dependent manner (PubMed:16354688). Plays an important role in regulating DNA replication and common fragile sites (CFS) stability in a RBBP6- and MCM10-dependent manner, represses expression of MCM10 which plays an important role in DNA-replication (PubMed:24726359). Acts as a transcriptional activator. May be involved in the differentiation and/or survival of late postmitotic neurons (By similarity). {ECO:0000250|UniProtKB:Q5EXX3, ECO:0000269|PubMed:16354688, ECO:0000269|PubMed:24726359}.

Molecular Weight: 134.3 kDa

UniProt: Q8NAP3