PLP1 Protein (AA 2-277)

Catalog No. ABIN3084135

Product Details

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Protein Type: Recombinant

Protein Characteristics: AA 2-277

Origin: Human

Source: Insect Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: MGLLECCARC L VGAPFASLVA TGLCFFGVAL FCGCGHEALT GTEKLIETYF SKNYQDYEYL INVIHAFQYV IYGTASFFFL YGALLLAEGF YTTGAVRQIF GDYKTTICGK GLSATVTGGQ KGRGSRGQHQ AHSLERVCHC LGKWLGHPDK FVGITYALTV VWLLVFACSA VPVYIYFNTW TTCQSIAFPS KTSASIGSLC ADARMYGVLP WNAFPGKVCG SNLLSICKTA EFQMTFHLFI AAFVGAAATL VSLLTFMIAA TYNFAVLKLM GRGTKFENLY

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using Rho1D4 capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In solution (20 mM Hepes, pH 7, 100 mM NaCl, 0,1 % OGNG)

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Alternative Name: PLP1 (PLP1 Products)

Synonyms: GPM6C Protein, HLD1 Protein, MMPL Protein, PLP Protein, PLP/DM20 Protein, PMD Protein, SPG2 Protein, Plp Protein, PLP1 Protein, plp1 Protein, DKFZp459O081 Protein, DKFZp459O113 Protein, DM20 Protein, jimpy Protein, jp Protein, msd Protein, rsh Protein, plp Protein, hld1 Protein, mmpl Protein, plp1a Protein, pmd Protein, spg2 Protein, DMalpha2c Protein, wu:fc27f01 Protein, wu:fj36d03 Protein, wu:fj42d08 Protein, zgc:110499 Protein, PLP-B Protein, plp1-a Protein, plp1-b Protein, plp1b Protein, proteolipid protein 1 Protein, proteolipid protein (myelin) 1 Protein, myelin proteolipid protein Protein, proteolipid protein 1 L homeolog Protein, proteolipid protein 1a Protein, proteolipid protein 1 S homeolog Protein, PLP1 Protein, Plp1 Protein, plp1 Protein, Tsp_11640 Protein, plp Protein, plp1.L Protein, plp1a Protein, plp1.S Protein

Background: This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.

Molecular Weight: 30.6 kDa

UniProt: P60201