Latrophilin 1 Protein (LPHN1) (AA 25-1474) (rho-1D4 tag)

Catalog No. ABIN3111030

Product Details

Target: Latrophilin 1 (LPHN1)

Protein Type: Recombinant

Protein Characteristics: AA 25-1474

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Latrophilin 1 protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: LSRAGLPFGL MRRELACEGY PIELRCPGSD VIMVENANYG RTDDKICDAD PFQMENVQCY LPDAFKIMSQ RCNNRTQCVV VAGSDAFPDP CPGTYKYLEV QYDCVPYKVE QKVFVCPGTL QKVLEPTSTH ESEHQSGAWC KDPLQAGDRI YVMPWIPYRT DTLTEYASWE DYVAARHTTT YRLPNRVDGT GFVVYDGAVF YNKERTRNIV KYDLRTRIKS GETVINTANY HDTSPYRWGG KTDIDLAVDE NGLWVIYATE GNNGRLVVSQ LNPYTLRFEG TWETGYDKRS ASNAFMVCGV LYVLRSVYVD DDSEAAGNRV DYAFNTNANR EEPVSLTFPN PYQFISSVDY NPRDNQLYVW NNYFVVRYSL EFGPPDPSAG PATSPPLSTT TTARPTPLTS TASPAATTPL RRAPLTTHPV GAINQLGPDL PPATAPVPST RRPPAPNLHV SPELFCEPRE VRRVQWPATQ QGMLVERPCP KGTRGIASFQ CLPALGLWNP RGPDLSNCTS PWVNQVAQKI KSGENAANIA SELARHTRGS IYAGDVSSSV KLMEQLLDIL DAQLQALRPI ERESAGKNYN KMHKRERTCK DYIKAVVETV DNLLRPEALE SWKDMNATEQ VHTATMLLDV LEEGAFLLAD NVREPARFLA AKENVVLEVT VLNTEGQVQE LVFPQEEYPR KNSIQLSAKT IKQNSRNGVV KVVFILYNNL GLFLSTENAT VKLAGEAGPG GPGGASLVVN SQVIAASINK ESSRVFLMDP VIFTVAHLED KNHFNANCSF WNYSERSMLG YWSTQGCRLV ESNKTHTTCA CSHLTNFAVL MAHREIYQGR INELLLSVIT WVGIVISLVC LAICISTFCF LRGLQTDRNT IHKNLCINLF LAELLFLVGI DKTQYEIACP IFAGLLHYFF LAAFSWLCLE GVHLYLLLVE VFESEYSRTK YYYLGGYCFP ALVVGIAAAI DYRSYGTEKA CWLRVDNYFI WSFIGPVSFV IVVNLVFLMV TLHKMIRSSS VLKPDSSRLD NIKSWALGAI ALLFLLGLTW AFGLLFINKE SVVMAYLFTT FNAFQGVFIF VFHCALQKKV HKEYSKCLRH SYCCIRSPPG GTHGSLKTSA MRSNTRYYTG TQSRIRRMWN DTVRKQTESS FMAGDINSTP TLNRGTMGNH LLTNPVLQPR GGTSPYNTLI AESVGFNPSS PPVFNSPGSY REPKHPLGGR EACGMDTLPL NGNFNNSYSL RSGDFPPGDG GPEPPRGRNL ADAAAFEKMI ISELVHNNLR GSSSAAKGPP PPEPPVPPVP GGGGEEEAGG PGGADRAEIE LLYKALEEPL LLPRAQSVLY QSDLDESESC TAEDGATSRP LSSPPGRDSL YASGANLRDS PSYPDSSPEG PSEALPPPPP APPGPPEIYY TSRPPALVAR NPLQGYYQVR RPSHEGYLAA PGLEGPGPDG DGQMQLVTSL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ADGRL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Latrophilin 1 (LPHN1)

Alternative Name: ADGRL1 (LPHN1 Products)

Synonyms: LPHN1 Protein, CIRL1 Protein, CL1 Protein, LEC2 Protein, 2900070I05Rik Protein, AI182398 Protein, CLIBA Protein, Lec2 Protein, mKIAA0821 Protein, CL1BA Protein, adhesion G protein-coupled receptor L1 Protein, ADGRL1 Protein, Adgrl1 Protein

Background: Calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization. Receptor propably implicated in the regulation of exocytosis (By similarity). {ECO:0000250}.

Molecular Weight: 161.4 kDa Including tag.

UniProt: O94910

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis