Phospholipase B Protein (PLB) (AA 22-1458) (rho-1D4 tag)

Catalog No. ABIN3115694

Product Details

Target: Phospholipase B (PLB)

Protein Type: Recombinant

Protein Characteristics: AA 22-1458

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Phospholipase B protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: QIHTSPRKST LEGQLWPETL KNSPFPCNPN KLGVNMPSKS VHSLKPSDIK FVAAIGNLEI PPDPGTGDLE KQDWTERPQQ VCMGVMTVLS DIIRYFSPSV PMPVCHTGKR VIPHDGAEDL WIQAQELVRN MKENLQLDFQ FDWKLINVFF SNASQCYLCP SAQQNGLAAG GVDELMGVLD YLQQEVPRAF VNLVDLSEVA EVSRQYHGTW LSPAPEPCNC SEETTRLAKV VMQWSYQEAW NSLLASSRYS EQESFTVVFQ PFFYETTPSL HSEDPRLQDS TTLAWHLWNR MMEPAGEKDE PLSVKHGRPM KCPSQESPYL FSYRNSNYLT RLQKPQDKLE VREGAEIRCP DKDPSDTVPT SVHRLKPADI NVIGALGDSL TAGNGAGSTP GNVLDVLTQY RGLSWSVGGD ENIGTVTTLA NILREFNPSL KGFSVGTGKE TSPNAFLNQA VAGGRAEDLP VQARRLVDLM KNDTRIHFQE DWKIITLFIG GNDLCDFCND LVHYSPQNFT DNIGKALDIL HAEVPRAFVN LVTVLEIVNL RELYQEKKVY CPRMILRSLC PCVLKFDDNS TELATLIEFN KKFQEKTHQL IESGRYDTRE DFTVVVQPFF ENVDMPKTSE GLPDNSFFAP DCFHFSSKSH SRAASALWNN MLEPVGQKTT RHKFENKINI TCPNQVQPFL RTYKNSMQGH GTWLPCRDRA PSALHPTSVH ALRPADIQVV AALGDSLTAG NGIGSKPDDL PDVTTQYRGL SYSAGGDGSL ENVTTLPNIL REFNRNLTGY AVGTGDANDT NAFLNQAVPG AKAEDLMSQV QTLMQKMKDD HRVNFHEDWK VITVLIGGSD LCDYCTDSNL YSAANFVHHL RNALDVLHRE VPRVLVNLVD FLNPTIMRQV FLGNPDKCPV QQASVLCNCV LTLRENSQEL ARLEAFSRAY RSSMRELVGS GRYDTQEDFS VVLQPFFQNI QLPVLADGLP DTSFFAPDCI HPNQKFHSQL ARALWTNMLE PLGSKTETLD LRAEMPITCP TQNEPFLRTP RNSNYTYPIK PAIENWGSDF LCTEWKASNS VPTSVHQLRP ADIKVVAALG DSLTTAVGAR PNNSSDLPTS WRGLSWSIGG DGNLETHTTL PNILKKFNPY LLGFSTSTWE GTAGLNVAAE GARARDMPAQ AWDLVERMKN SPDINLEKDW KLVTLFIGVN DLCHYCENPE AHLATEYVQH IQQALDILSE ELPRAFVNVV EVMELASLYQ GQGGKCAMLA AQNNCTCLRH SQSSLEKQEL KKVNWNLQHG ISSFSYWHQY TQREDFAVVV QPFFQNTLTP LNERGDTDLT FFSEDCFHFS DRGHAEMAIA LWNNMLEPVG RKTTSNNFTH SRAKLKCPSP ESPYLYTLRN SRLLPDQAEE APEVLYWAVP VAAGVGLVVG IIGTVVWRCR RGGRREDPPM SLRTVAL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PLB1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Phospholipase B (PLB)

Alternative Name: PLB1 (PLB Products)

Synonyms: CMD1P Protein, CMH18 Protein, PLB Protein, PLB/LIP Protein, hPLB Protein, Phlpb Protein, 4632413E21Rik Protein, 4930433E17Rik Protein, 4930539A06Rik Protein, BC033606 Protein, PLB1 Protein, PHLPB Protein, phospholamban Protein, phospholipase B1 Protein, phospholipase B Protein, PLN Protein, PLB1 Protein, Plb1 Protein

Background: Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity). {ECO:0000250}.

Molecular Weight: 162.0 kDa Including tag.

UniProt: Q6P1J6