KCNT2 Protein (AA 1-1135) (Strep Tag)

Catalog No. ABIN3115770

Product Details

Target: KCNT2 (Potassium Channel, Subfamily T, Member 2 (KCNT2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1135

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This KCNT2 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MVDLESEVPP LPPRYRFRDL LLGDQGWQND DRVQVEFYMN ENTFKERLKL FFIKNQRSSL RIRLFNFSLK LLSCLLYIIR VLLENPSQGN EWSHIFWVNR SLPLWGLQVS VALISLFETI LLGYLSYKGN IWEQILRIPF ILEIINAVPF IISIFWPSLR NLFVPVFLNC WLAKHALENM INDLHRAIQR TQSAMFNQVL ILISTLLCLI FTCICGIQHL ERIGKKLNLF DSLYFCIVTF STVGFGDVTP ETWSSKLFVV AMICVALVVL PIQFEQLAYL WMERQKSGGN YSRHRAQTEK HVVLCVSSLK IDLLMDFLNE FYAHPRLQDY YVVILCPTEM DVQVRRVLQI PMWSQRVIYL QGSALKDQDL LRAKMDDAEA CFILSSRCEV DRTSSDHQTI LRAWAVKDFA PNCPLYVQIL KPENKFHIKF ADHVVCEEEF KYAMLALNCI CPATSTLITL LVHTSRGQEG QQSPEQWQKM YGRCSGNEVY HIVLEESTFF AEYEGKSFTY ASFHAHKKFG VCLIGVRRED NKNILLNPGP RYIMNSTDIC FYINITKEEN SAFKNQDQQR KSNVSRSFYH GPSRLPVHSI IASMGTVAID LQDTSCRSAS GPTLSLPTEG SKEIRRPSIA PVLEVADTSS IQTCDLLSDQ SEDETTPDEE MSSNLEYAKG YPPYSPYIGS SPTFCHLLHE KVPFCCLRLD KSCQHNYYED AKAYGFKNKL IIVAAETAGN GLYNFIVPLR AYYRPKKELN PIVLLLDNPP DMHFLDAICW FPMVYYMVGS IDNLDDLLRC GVTFAANMVV VDKESTMSAE EDYMADAKTI VNVQTLFRLF SSLSIITELT HPANMRFMQF RAKDCYSLAL SKLEKKERER GSNLAFMFRL PFAAGRVFSI SMLDTLLYQS FVKDYMISIT RLLLGLDTTP GSGFLCSMKI TADDLWIRTY ARLYQKLCSS TGDVPIGIYR TESQKLTTSE SQISISVEEW EDTKDSKEQG HHRSNHRNST SSDQSDHPLL RRKSMQWARR LSRKGPKHSG KTAEKITQQR LNLYRRSERQ ELAELVKNRM KHLGLSTVGY DEMNDHQSTL SYILINPSPD TRIELNDVVY LIRPDPLAYL PNSEPSRRNS ICNVTGQDSR EETQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KCNT2 (Potassium Channel, Subfamily T, Member 2 (KCNT2))

Alternative Name: KCNT2 (KCNT2 Products)

Synonyms: KCa4.2 Protein, SLICK Protein, SLO2.1 Protein, E330038N15Rik Protein, Slick Protein, potassium sodium-activated channel subfamily T member 2 Protein, potassium channel subfamily T member 2 Protein, potassium channel, subfamily T, member 2 Protein, KCNT2 Protein, LOC100541564 Protein, kcnt2 Protein, Kcnt2 Protein

Background: Potassium channel subfamily T member 2 (Sequence like an intermediate conductance potassium channel subunit) (Sodium and chloride-activated ATP-sensitive potassium channel Slo2.1),FUNCTION: Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. Activated by high intracellular sodium and chloride levels (PubMed:14684870, PubMed:16687497, PubMed:29069600). Channel activity is inhibited by ATP and by inhalation anesthetics, such as isoflurane (PubMed:16687497) (By similarity). Inhibited upon stimulation of G-protein coupled receptors, such as CHRM1 and GRM1 (PubMed:16687497). {ECO:0000250|UniProtKB:Q6UVM4, ECO:0000269|PubMed:14684870, ECO:0000269|PubMed:16687497, ECO:0000269|PubMed:29069600}.

Molecular Weight: 130.5 kDa

UniProt: Q6UVM3