ASXL2 Protein (AA 1-1435) (Strep Tag)

Catalog No. ABIN3115953

Product Details

Target: ASXL2 (Additional Sex Combs Like 2 (ASXL2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1435

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ASXL2 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MREKGRRKKG RTWAEAAKTV LEKYPNTPMS HKEILQVIQR EGLKEIRSGT SPLACLNAML HTNSRGEEGI FYKVPGRMGV YTLKKDVPDG VKELSEGSEE SSDGQSDSQS SENSSSSSDG GSNKEGKKSR WKRKVSSSSP QSGCPSPTIP AGKVISPSQK HSKKALKQAL KQQQQKKQQQ QCRPSISISS NQHLSLKTVK AASDSVPAKP ATWEGKQSDG QTGSPQNSNS SFSSSVKVEN TLLGLGKKSF QRSERLHTRQ MKRTKCADID VETPDSILVN TNLRALINKH TFSVLPGDCQ QRLLLLLPEV DRQVGPDGLM KLNGSALNNE FFTSAAQGWK ERLSEGEFTP EMQVRIRQEI EKEKKVEPWK EQFFESYYGQ SSGLSLEDSK KLTASPSDPK VKKTPAEQPK SMPVSEASLI RIVPVVSQSE CKEEALQMSS PGRKEECESQ GEVQPNFSTS SEPLLSSALN THELSSILPI KCPKDEDLLE QKPVTSAEQE SEKNHLTTAS NYNKSESQES LVTSPSKPKS PGVEKPIVKP TAGAGPQETN MKEPLATLVD QSPESLKRKS SLTQEEAPVS WEKRPRVTEN RQHQQPFQVS PQPFLNRGDR IQVRKVPPLK IPVSRISPMP FHPSQVSPRA RFPVSITSPN RTGARTLADI KAKAQLVKAQ RAAAAAAAAA AAAASVGGTI PGPGPGGGQG PGEGGEGQTA RGGSPGSDRV SETGKGPTLE LAGTGSRGGT RELLPCGPET QPQSETKTTP SQAQPHSVSG AQLQQTPPVP PTPAVSGACT SVPSPAHIEK LDNEKLNPTR ATATVASVSH PQGPSSCRQE KAPSPTGPAL ISGASPVHCA ADGTVELKAG PSKNIPNPSA SSKTDASVPV AVTPSPLTSL LTTATLEKLP VPQVSATTAP AGSAPPSSTL PAASSLKTPG TSLNMNGPTL RPTSSIPANN PLVTQLLQGK DVPMEQILPK PLTKVEMKTV PLTAKEERGM GALIATNTTE NSTREEVNER QSHPATQQQL GKTLQSKQLP QVPRPLQLFS AKELRDSSID THQYHEGLSK ATQDQILQTL IQRVRRQNLL SVVPPSQFNF AHSGFQLEDI STSQRFMLGF AGRRTSKPAM AGHYLLNIST YGRGSESFRR THSVNPEDRF CLSSPTEALK MGYTDCKNAT GESSSSKEDD TDEESTGDEQ ESVTVKEEPQ VSQSAGKGDT SSGPHSRETL STSDCLASKN VKAEIPLNEQ TTLSKENYLF TRGQTFDEKT LARDLIQAAQ KQMAHAVRGK AIRSSPELFS STVLPLPADS PTHQPLLLPP LQTPKLYGSP TQIGPSYRGM INVSTSSDMD HNSAVPGSQV SSNVGDVMSF SVTVTTIPAS QAMNPSSHGQ TIPVQAFSEE NSIEGTPSKC YCRLKAMIMC KGCGAFCHDD CIGPSKLCVS CLVVR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ASXL2 (Additional Sex Combs Like 2 (ASXL2))

Alternative Name: ASXL2 (ASXL2 Products)

Synonyms: asxh2 Protein, ASXH2 Protein, 4930556B16Rik Protein, mKIAA1685 Protein, additional sex combs like 2, transcriptional regulator Protein, additional sex combs like 2 (Drosophila) Protein, Asxl2 Protein, ASXL2 Protein, asxl2 Protein

Background: Putative Polycomb group protein ASXL2 (Additional sex combs-like protein 2),FUNCTION: Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via methylation of histones, rendering chromatin heritably changed in its expressibility (By similarity). Involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as peroxisome proliferator-activated receptor gamma (PPARG). Acts as coactivator for PPARG and enhances its adipocyte differentiation-inducing activity, the function seems to involve differential recruitment of acetylated and methylated histone H3. {ECO:0000250, ECO:0000269|PubMed:21047783}.

Molecular Weight: 153.8 kDa

UniProt: Q76L83

Pathways: Positive Regulation of fat Cell Differentiation