GPR125 Protein (AA 34-1321) (rho-1D4 tag)

Catalog No. ABIN3116338

Product Details

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Protein Type: Recombinant

Protein Characteristics: AA 34-1321

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This GPR125 protein is labelled with rho-1D4 tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: GGAAALPAGC KHDGRPRGAG RAAGAAEGKV VCSSLELAQV LPPDTLPNRT VTLILSNNKI SELKNGSFSG LSLLERLDLR NNLISSIDPG AFWGLSSLKR LDLTNNRIGC LNADIFRGLT NLVRLNLSGN LFSSLSQGTF DYLASLRSLE FQTEYLLCDC NILWMHRWVK EKNITVRDTR CVYPKSLQAQ PVTGVKQELL TCDPPLELPS FYMTPSHRQV VFEGDSLPFQ CMASYIDQDM QVLWYQDGRI VETDESQGIF VEKNMIHNCS LIASALTISN IQAGSTGNWG CHVQTKRGNN TRTVDIVVLE SSAQYCPPER VVNNKGDFRW PRTLAGITAY LQCTRNTHGS GIYPGNPQDE RKAWRRCDRG GFWADDDYSR CQYANDVTRV LYMFNQMPLN LTNAVATARQ LLAYTVEAAN FSDKMDVIFV AEMIEKFGRF TKEEKSKELG DVMVDIASNI MLADERVLWL AQREAKACSR IVQCLQRIAT YRLAGGAHVY STYSPNIALE AYVIKSTGFT GMTCTVFQKV AASDRTGLSD YGRRDPEGNL DKQLSFKCNV SNTFSSLALK NTIVEASIQL PPSLFSPKQK RELRPTDDSL YKLQLIAFRN GKLFPATGNS TNLADDGKRR TVVTPVILTK IDGVNVDTHH IPVNVTLRRI AHGADAVAAR WDFDLLNGQG GWKSDGCHIL YSDENITTIQ CYSLSNYAVL MDLTGSELYT QAASLLHPVV YTTAIILLLC LLAVIVSYIY HHSLIRISLK SWHMLVNLCF HIFLTCVVFV GGITQTRNAS ICQAVGIILH YSTLATVLWV GVTARNIYKQ VTKKAKRCQD PDEPPPPPRP MLRFYLIGGG IPIIVCGITA AANIKNYGSR PNAPYCWMAW EPSLGAFYGP ASFITFVNCM YFLSIFIQLK RHPERKYELK EPTEEQQRLA ANENGEINHQ DSMSLSLIST SALENEHTFH SQLLGASLTL LLYVALWMFG ALAVSLYYPL DLVFSFVFGA TSLSFSAFFV VHHCVNREDV RLAWIMTCCP GRSSYSVQVN VQPPNSNGTN GEAPKCPNSS AESSCTNKSA SSFKNSSQGC KLTNLQAAAA QCHANSLPLN STPQLDNSLT EHSMDNDIKM HVAPLEVQFR TNVHSSRHHK NRSKGHRASR LTVLREYAYD VPTSVEGSVQ NGLPKSRLGN NEGHSRSRRA YLAYRERQYN PPQQDSSDAC STLPKSSRNF EKPVSTTSKK DALRKPAVVE LENQQKSYGL NLAIQNGPIK SNGQEGPLLG TDSTGNVRTG LWKHETTV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ADGRA3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Alternative Name: ADGRA3 (GPR125 Products)

Synonyms: PGR21 Protein, TEM5L Protein, 3830613O22Rik Protein, AU044632 Protein, Tem5-like Protein, GPR125 Protein, Gpr125 Protein, adhesion G protein-coupled receptor A3 Protein, adhesion G protein-coupled receptor A3-like Protein, ADGRA3 Protein, Adgra3 Protein, ADGRA3L Protein, adgra3 Protein

Background: Orphan receptor that may have a role in planar cell polarity pathway. {ECO:0000250|UniProtKB:S4X0Q8}.

Molecular Weight: 144.0 kDa Including tag.

UniProt: Q8IWK6