SPPL3 Protein (AA 1-385) (rho-1D4 tag)

Catalog No. ABIN3116818

Product Details

Target: SPPL3 (Signal Peptide Peptidase 3 (SPPL3))

Protein Type: Recombinant

Protein Characteristics: AA 1-385

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This SPPL3 protein is labelled with rho-1D4 tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: MAEQTYSWAY SLVDSSQVST FLISILLIVY GSFRSLNMDF ENQDKEKDSN SSSGSFNGEQ EPIIGFQPMD STRARFLPMG ACVSLLVMFF FFDSVQVVFT ICTAVLATIA FAFLLLPMCQ YLTRPCSPQN KISFGCCGRF TAAELLSFSL SVMLVLIWVL TGHWLLMDAL AMGLCVAMIA FVRLPSLKVS CLLLSGLLIY DVFWVFFSAY IFNSNVMVKV ATQPADNPLD VLSRKLHLGP NVGRDVPRLS LPGKLVFPSS TGSHFSMLGI GDIVMPGLLL CFVLRYDNYK KQASGDSCGA PGPANISGRM QKVSYFHCTL IGYFVGLLTA TVASRIHRAA QPALLYLVPF TLLPLLTMAY LKGDLRRMWS EPFHSKSSSS RFLEV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SPPL3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SPPL3 (Signal Peptide Peptidase 3 (SPPL3))

Alternative Name: SPPL3 (SPPL3 Products)

Synonyms: CG17370 Protein, CG5901 Protein, Dmel\\CG17370 Protein, SPPL3 Protein, Sppl Protein, sppL Protein, RGD1562826 Protein, Sppl3-ps1 Protein, MGC52975 Protein, sppl3 Protein, MGC75937 Protein, fb94d08 Protein, wu:fb94d08 Protein, zgc:114093 Protein, 4833416I09Rik Protein, Psl4 Protein, Usmg3 Protein, IMP2 Protein, MDHV1887 Protein, PRO4332 Protein, PSH1 Protein, PSL4 Protein, Signal peptide peptidase-like Protein, signal peptide peptidase like 3 Protein, signal peptide peptidase like 3 L homeolog Protein, signal peptide peptidase 3 Protein, Intramembrane protease 2 Protein, SppL Protein, Sppl3 Protein, sppl3.L Protein, sppl3 Protein, SPPL3 Protein, imp-2 Protein

Background: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries (PubMed:16873890, PubMed:25354954, PubMed:25827571). Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Catalyzes the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis (PubMed:23132852). May also have the ability to serve as a shedding protease for subsequent intramembrane proteolysis by SPPL2A and SPPL2B of the envelope glycoprotein gp130 (PubMed:23132852). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971). {ECO:0000269|PubMed:16873890, ECO:0000269|PubMed:23132852, ECO:0000269|PubMed:25354954, ECO:0000269|PubMed:25384971, ECO:0000269|PubMed:25827571}.

Molecular Weight: 43.7 kDa Including tag.

UniProt: Q8TCT6