MUC3B Protein (AA 22-1237) (rho-1D4 tag)
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- Target See all MUC3B products
- MUC3B (Mucin 3B, Cell Surface Associated (MUC3B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-1237
- Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This MUC3B protein is labelled with rho-1D4 tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- Sequence
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TLSTATSTSH VTFPRAEATR TALSNSPHSR YLAEWPQGVP QLASPAPGHR ENAPMTLTTS PHDTLISETL LSSLVSSNTS TTPTSKFAFK VETTPPTVLV YSATTECVYP TSFIITISHS TSICVTTTQV TFTSSYTPTP VTQKPVTTVT RTYPMTTTEK GTSAMISSPS TTTARETPIV TVTPSSSVSA TDTTFHTTIS STTRTTERTP LPTGSIHTTM SPTPVFTTLK TAVTSTSPIT STITSTNTVT SMTTTTSRPT ATNTLSSLTS SILSSTPAPN TEVITSHTTT TTPPSTLVTT LPTAIARSTP TSETTPSFMS SIITTETTSH STPSFSSSTI HSTVSSSTTA ITSPFTTAET GVTSTPSSPS SLSTDIPTTS LRTLTPLSLS TSTSLTTTTD LPSIPTDISS LPTPIHIISS SPSIQSTETS SLVGTTSPTM STVRATLRST ENTPISSFST SIVVTPETPT TQAPPVLMSA TGTQTSPVPT TVTFGSMDSS TSTLHTLTPS TALSKIMSTS QFPIPSTHSS TLQTTPSIPS LQTSLTSTSE FTTESFTRGS TSTNAILTSF STIIWSSTPT IIMSSSPSSA SITPVFATTI HSVPSSPYIF STENVGSASI TAFPSLSSSS TTSTSPTSSS LTTALTEITP FSYISLPSTT PCPGTITITI VPASPTDPCV EMDPSTEATS PPTTPLTVFP FTTEMVTCPS SISMQTTLAT HMDTSSMTPE SESSIIPNAS SSTGTGTVPT NTVFTSTRLP TSETWLSNNS VIPTPLPGVS TIPLTMKPSS SLPTILRTSS KSTHPSPPTA RTSQTSVATT QTPTTLTTCR TTPITSWMTT QSTLTTTAGT CDNGGTWEQG QCACLPGFSG DRCQLQTRCQ NGGQWDGLKC QCPSTFYGSS CEFAVEQVDL DVVETEVGME VSVDQQFSPD LNDNTSQAYR DFNKTFWNQM QKIFADMQGF TFKGVEILSL RNGSIVVDYL VLLEMPFSPQ LESEYEQVKT TLKEGLQNAS QDANSCQDSQ ALCFKPDSIK VNNNSKTELT PEAICRRAAP TGYEEFYFPL VEATRLRCVT KCTSGLDNAI DCHQGQCVLE TSGPACRCYS TDTHWFSGPR CEVTVHWRAL VGGLTAGAAL LVLLLLALGV RAVRSEWWGR QRRGRSWDQD RKWFETWDEE VVGTFSNWGF EDDGTDKDTN FHVALENVDT TMKVHIKRPE MTSSSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MUC3B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- MUC3B (Mucin 3B, Cell Surface Associated (MUC3B))
- Alternative Name
- MUC3B (MUC3B Products)
- Synonyms
- MUC3 Protein, MUC3A Protein, MUC3B Protein, mucin 3B, cell surface associated Protein, mucin-3B Protein, MUC3B Protein, LOC463613 Protein
- Background
- Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (By similarity). {ECO:0000250}.
- Molecular Weight
- 130.4 kDa Including tag.
- UniProt
- Q9H195
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