PHF16 Protein (AA 1-823) (Strep Tag)

Catalog No. ABIN3123499

Product Details

Target: PHF16 (PHD Finger Protein 16 (PHF16))

Protein Type: Recombinant

Protein Characteristics: AA 1-823

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PHF16 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MKRHRPVSSS ESSDECPSTS FTSSSMYRKK SKNPKEQKKS AEVFRKDLIS AMKIPDSHHV NPDSYYLFTD TWKEEWEKGV QVPANPDSVP TPSLRIISEK VKEMLFVRPR KYIRCSSPES AEPGYINTLE QAASTCRYDL DDMDIFWLQE LNEDLGEMGY GPIDETLMEK TIEVLERHCH ENMNHAIETV EGLGIEYDED VICDVCRSPD SEEGNDMVFC DKCNVCVHQA CYGILKIPEG SWLCRSCVLG IYPQCVLCPK KGGAMKTTRT GTKWAHVSCA LWIPEVSIAC PERMEPVTKI SHIPPSRWAL VCNLCKLKTG ACIQCSVKSC ITAFHVTCAF EHGLEMKTIL DEGDEVKFKS FCLKHSQNKP KLGDAEYHHH RVAEQSQAKS EKTSLRAQKL RELEEEFYTL VQVEDVAKEM ELSAFTVDFI YNYWKLKRKS NFNKPLIPPK EEEENGLVQP KEESIHTRMR MFMHLRQDLE RVRNLCYMIS RREKLKLSHT KVQEQIFGLQ VQLINEEITE GLSLTNALEN SLFYPPPRIT LKLKMPKSTS EDCKDSSTET EHQLSSPGSS SPGHSKRSPQ MPEEPLDMNV KIYPRYPLES KSNCLQTSRS HSRCETKSSS PTPRAPSAEF YHGQSLGKPL ALQAALHGQV SIGNGKNQPN SRVSSSNGLE GNWSGNITQK VNSSEVCYDQ ESMLSSHLPS PGNIRKSSME HFSRSFKEAT NTWVKPTEDL QYCVKPTKNV SSKEQLWGRQ LLRRPTGRAS YQETDGYCPD LEPSDSEAEG EGSKETPRVK RESSDRENPS HDSARECHGK TKTHPHSHSS MQR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PHF16 (PHD Finger Protein 16 (PHF16))

Alternative Name: Jade3 (PHF16 Products)

Synonyms: JADE3 Protein, 5730598B06 Protein, AI851988 Protein, AW544806 Protein, Jade3 Protein, mKIAA0215 Protein, jade3 Protein, wu:fb53c02 Protein, jade family PHD finger 3 Protein, JADE3 Protein, Jade3 Protein, jade3 Protein

Background: Protein Jade-3 (Jade family PHD finger protein 3) (PHD finger protein 16),FUNCTION: Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity. {ECO:0000250|UniProtKB:Q92613}.

Molecular Weight: 93.5 kDa

UniProt: Q6IE82