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BUD31 Protein (AA 1-103) (His tag)

Crystallography grade BUD31 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3123751
  • Target See all BUD31 Proteins
    BUD31 (BUD31 Homolog (BUD31))
    Protein Type
    Protein Characteristics
    AA 1-103
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 6
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This BUD31 protein is labelled with His tag.
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Bud31 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Crystallography grade
    Top Product
    Discover our top product BUD31 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    For Research Use only
  • Format
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    BUD31 (BUD31 Homolog (BUD31))
    Alternative Name
    Bud31 (BUD31 Products)
    Cwc14 Protein, EDG-2 Protein, EDG2 Protein, G10 Protein, YCR063W Protein, fSAP17 Protein, g10l Protein, zgc:110615 Protein, CWC14 Protein, DDBDRAFT_0190998 Protein, DDBDRAFT_0233523 Protein, DDB_0190998 Protein, DDB_0233523 Protein, bud31 Protein, Edg2 Protein, C77604 Protein, g10 Protein, c77604 Protein, BUD31 homolog Protein, BUD31 homolog (S. cerevisiae) Protein, protein BUD31 homolog Protein, U2 snRNP complex subunit BUD31 Protein, U2 snRNP complex subunit Protein, hypothetical protein Protein, BUD31 homolog (yeast) Protein, BUD31 homolog L homeolog Protein, maternal g10 transcript Protein, Protein BUD31 homolog Protein, BUD31 Protein, bud31 Protein, Bud31 Protein, bud31.L Protein, LOC5569643 Protein, CC1G_08861 Protein, bud-31 Protein, LOC109464260 Protein, LOC108703088 Protein
    Molecular Weight
    12.9 kDa Including tag.
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