CCDC135 Protein (AA 1-876) (Strep Tag)

Catalog No. ABIN3123850

Product Details

Target: CCDC135 (Coiled-Coil Domain Containing 135 (CCDC135))

Protein Type: Recombinant

Protein Characteristics: AA 1-876

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CCDC135 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MEVLREKVEE EEEAEREEAA ERAERTEKLE RVTKSAEVSR EGTILSQDEL RDLEGKLMAI EIPTQADHSV ISQAPVDVTK LPPSYTTNSL KEEHLLLVAD NFSRQYSHLC PDRVPLFLHP LNECNVPKFV STTLRPTLMP YPELYNWDSC AQFVSDFLTM VPLVDPLKPP THLYSSTTVL KCQKGNCFDF STLLCSMLIG SGYDAYCVNG YGSLDLCLMD LTREVCPLTV KAKEIVKKKE KTVPKKYSIK PPRDLTSKFE QEQEEKRIQE IKDLEQRRLK EEEDRILEAE KAKPDPLHGL RVHSWVLVLA GKREVPESFF IDPLTARSYS TKDEHFLGIE SLWNHKNYWI NMQDCWNCCK DLIFDLGDPV RWEYMLLGTD KPHLSLTEED EEGLDDDDDD VEDLGKEEED KSFDMPSSWV SQIEITPEEF ETRCPSGKKV IQYKKAQLEK WSPYLNNNGL VCRLTTYEDQ QCTKVLEIKE WYQNREDMLE LKHINKTTGL HVDYFKPGHP QALCVHSYKS MLPEMDRVME FYKKIRVDGL VKREETPMTM TEYYEGRSDF LAYRHVNFGP RVKKLSQSSV ESNPRPMVKI TERFSRNPEK PADEDVAERL FLIVEERIQL RYHCRDDYIT ASKREFLRRM EVDSKGNKII MTPEMCISYE VEPMEHTKKL LYQYETMNQL KNEEKLSRHQ AWESELEVLE ILKLREEEEE AHTLTISIYD TKRNEKSKEY REAMERVLHE EHLRQVEAQL DYLAPFLAQL PPGEKLTRWQ AVRLKDECLS DFKQRLIDKA NLIQARFEKE TQELQKKQQW YQENQVTLTP EDENLYLSYC SQAMFRIRIL EQRLNRHKEL APLKYLALEE KLYKDPRLID FVKVFV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CCDC135 (Coiled-Coil Domain Containing 135 (CCDC135))

Alternative Name: Drc7 (CCDC135 Products)

Synonyms: RGD1565601 Protein, MGC146237 Protein, C16orf50 Protein, FAP50 Protein, Gm770 Protein, SRG-L Protein, SRG-S Protein, dynein regulatory complex subunit 7 Protein, flagellar associated protein Protein, Drc7 Protein, DRC7 Protein, drc7 Protein, LOC100181033 Protein, FAP50 Protein

Background: Dynein regulatory complex subunit 7 (Coiled-coil domain-containing protein 135) (Coiled-coil domain-containing protein lobo homolog) (Spermatogenesis-related gene in late stages of spermatogenesis cells protein),FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC) a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes (By similarity). Essential for male fertility, sperm head morphogenesis and sperm flagellum formation (PubMed:31961863). Not required for ciliogenesis in the brain and trachea (PubMed:31961863). {ECO:0000250|UniProtKB:A8JAM0, ECO:0000269|PubMed:31961863}.

Molecular Weight: 103.3 kDa

UniProt: Q6V3W6