DZIP1 Protein (AA 1-852) (Strep Tag)

Catalog No. ABIN3125040

Product Details

Target: DZIP1 (DAZ Interacting Protein 1 (DZIP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-852

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DZIP1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MPFQKHVYYP LANSPEGPDA SAIGAAPMAF VPPSAASGPL PFFQFRPRLE SVDWRRLSAI DVDKVAGAVD VLTLQENIMN ITFCKLEDEK CPHCQSGVDP VLLKLIRLAQ LTIEYLMHSQ EFLTSQLNLV EERLRLSLLD YEQSKQLLTK QAGEIKLLKE ECKRRKKMLS TQQLMIEAKA SYYQCHFCDK AFMNQAFLQS HIQRRHTEDS HLEYNTKAQT DRLQKEIDML KEQLQLTRSQ LESAQHSHAV RFSKDYEMQK SKEEDFLKLF DRWKEEEKEK LLEEMEKVKG MFMREFKELT SKNSALEYQL LEIQKSNIQI KSNIGTLRDV TELREDHLPC PQDFQNMLQL LDSQASKWTD RFQVLNEEHS KEKGQLLSHI EKLRSSMMKD LSADNVFYKR RVEELGQKLQ EQNELIISQK QQIREFASKP YSSISELKGT PLTRQTLEPK SAAPTTPMTA SATQNLDGAS SLTMVHEQVF SSHILEPIEE LSSEEEKGRE NEQKLNKKTS LRKPSSTSPS PQELRTNLER ELGNKLRSFG IGANIQGIPC EILNRSLKAM QVARHDLAKQ MPDIQQIRES LEHQLICKME EKVSLSSDRH HVPSMTTFPP EEVPKATQLP HKSRPLVRQR TVFTDKVSVP KLKKNTKESH FLRRFPSTKT PPFSSEEEPD EEDLLHAYLS PDSLATAATQ PPKSSMSHFG KSAVKSDTDW TEGSEMDDSD FSPKLTGTSI TIQTDTVETM ALPQGSGNKA VPGMNPADTV IKKESLQELK CTDADDEDWD ISSLEEEKSL GSKIEQREPP PAKRDPSCTQ VQRAWGPVNP REFKEEGLHE NEPSTLKSNL VTVTDWSDVL DV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DZIP1 (DAZ Interacting Protein 1 (DZIP1))

Alternative Name: Dzip1 (DZIP1 Products)

Synonyms: dzip Protein, dzipt1 Protein, DZIP1 Protein, MGC142440 Protein, MGC146244 Protein, rp11-23e3.3 Protein, 2510025K24Rik Protein, 2810422M04Rik Protein, mKIAA0996 Protein, DZIP Protein, DZIPt1 Protein, igu Protein, igu/dzip1 Protein, si:dz52i16.1 Protein, DAZ interacting zinc finger protein 1 Protein, DAZ interacting zinc finger protein 1 L homeolog Protein, DAZ interacting protein 1 Protein, DZIP1 Protein, dzip1.L Protein, dzip1 Protein, Dzip1 Protein

Background: Cilium assembly protein DZIP1 (DAZ-interacting protein 1 homolog) (DAZ-interacting zinc finger protein 1),FUNCTION: Molecular adapter that recruits protein complexes required for cilium assembly and function to the cilium basal body (PubMed:23955340, PubMed:25860027, PubMed:31118289, PubMed:32051257). At the exit of mitosis, localizes to the basal body and ciliary base of the forming primary cilium where it recruits and activates RAB8A to direct vesicle-mediated transport of proteins to the cilium (PubMed:25860027). Also recruits the BBSome, a complex involved in cilium biogenesis, by bridging it to PCM1 at the centriolar satellites of the cilium (PubMed:27979967). It is also required for the recruitment to the cilium basal body of the intraflagellar transport (IFT) machinery as well as the ciliary appendage proteins CEP164 and NINEIN (PubMed:23955340). Functions as a regulator of Hedgehog signaling both through its role in cilium assembly but also probably through its ability to retain GLI3 within the cytoplasm (PubMed:23955340). It is involved in spermatogenesis through its role in organization of the basal body and assembly of the sperm flagellum (PubMed:32051257). Also indirectly involved in heart development through its function in ciliogenesis (PubMed:31118289). {ECO:0000269|PubMed:23955340, ECO:0000269|PubMed:25860027, ECO:0000269|PubMed:27979967, ECO:0000269|PubMed:31118289, ECO:0000269|PubMed:32051257}.

Molecular Weight: 97.3 kDa

UniProt: Q8BMD2

Pathways: Hedgehog Signaling, Protein targeting to Nucleus