Epsin 2 Protein (EPN2) (AA 1-595) (Strep Tag)

Catalog No. ABIN3126044

Product Details

Target: Epsin 2 (EPN2)

Protein Type: Recombinant

Protein Characteristics: AA 1-595

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Epsin 2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV VAFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KVERVQALKT KERMAQVATG VGSNQITFGR GSSQPNLSTS YSEQEYGKAG GSPASYHGST SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQSSES VQTARGSKEE RLRRGDDLRL QMALEESRRD TVKVPKKKEA KACCKPGSHS QQTTLLDLMD ALPSSGPVTQ KTEPWSAGAS ANQTNPWGGT VAPSNITDPW PSFGTKPAAS VDPWGVPTTA STQSVPKNSD PWAASQQPAS NAGKTTDAWG AAKPSSASGS FELFSNFNGT VKDDFSEFDN LRTSKKPAES GASVPPQDSR TTSPDLFESQ SLTSASSKPS SARKTPESFL GPNAALVNLD SLVTKPAPPA QSLNPFLAPG AAAPAPVNPF QVNQPQPLTL NQLRGSPVLG SSASFGSGPG VETVAPMTSV APHSSVGASG SSLTPLGPTA MNMVGSVGIP PSAAQSTGTT NPFLL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Epsin 2 (EPN2)

Alternative Name: Epn2 (EPN2 Products)

Synonyms: CG13853 Protein, CG13854 Protein, CG31170 Protein, CG31285 Protein, CG42250 Protein, Dmel\\CG42250 Protein, Dmel_CG31170 Protein, Dmel_CG31285 Protein, Epsin Protein, LqfR Protein, XII-10 Protein, epsin Protein, epsin-like Protein, epsin02 Protein, epsinR Protein, l(3)03685 Protein, l(3)A9 Protein, l(3)SG62 Protein, l(3)XII-10 Protein, l(3)dsl-9 Protein, l(3)dsl9 Protein, EHB21 Protein, 9530051D10Rik Protein, AA536924 Protein, Ibp2 Protein, epsin-2 Protein, liquid facets-Related Protein, epsin 2 Protein, epsin-2 Protein, Epsin 2 Protein, epsin 2 S homeolog Protein, lqfR Protein, EPN2 Protein, EDI_326810 Protein, EDI_044850 Protein, Bm1_38210 Protein, EHI_104950 Protein, epn2 Protein, Epn2 Protein, epn2.S Protein

Background: Epsin-2 (EPS-15-interacting protein 2) (Intersectin-EH-binding protein 2) (Ibp2),FUNCTION: Plays a role in the formation of clathrin-coated invaginations and endocytosis. {ECO:0000250}.

Molecular Weight: 63.5 kDa

UniProt: Q8CHU3

Pathways: EGFR Downregulation