PAIP1 Protein (AA 1-400) (Strep Tag)

Catalog No. ABIN3127048

This Recombinant PAIP1 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for PAIP1 Protein (AA 1-400) (Strep Tag) (ABIN3127048)

Target: PAIP1 (Poly(A) Binding Protein Interacting Protein 1 (PAIP1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-400

Purification tag / Conjugate: This PAIP1 protein is labelled with Strep Tag.

Sequence: MSDSFDRAPE QTKPQRAPPS SQDKIPQQNS ESAMAKPQVV VAPVLMSKLS ANAPEFYPSG YSSNYTESYE DGCEDYPTLS EYVQDFLNHL TEQPGSFETE IEQFAETLNG WVTTDDALQE LVELIYQQAT SIPNFSYMGA RLCNYLSHHL TISPQSGNFR QLLLQRCRTE YEAKDQAAKG DEVTRKRFHA FVLFLGELYL NLEIKGTNGQ VTRADILQVG LRELLNALFS NPMDDNLICA VKLLKLTGSV LEDTWKEKGK TDMEEIIQRI ENVVLDANCS RDVKQMLLKL VELRSSNWGR VHATSTYREA TPENDPNYFM NEPTFYTSDG VPFTAADPDY QEKYQELLER EDFFPDYEEN GTDLSGAGDP YLDDIDDEMD PEIEEAYEKF CLESERKRKQ
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PAIP1 (Poly(A) Binding Protein Interacting Protein 1 (PAIP1))

Alternative Name: Paip1

Background: Polyadenylate-binding protein-interacting protein 1 (PABP-interacting protein 1) (PAIP-1) (Poly(A)-binding protein-interacting protein 1),FUNCTION: Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (By similarity). {ECO:0000250}.

Molecular Weight: 45.7 kDa

UniProt: Q8VE62