NOVA1 Protein (AA 1-507) (Strep Tag)

Catalog No. ABIN3130250

Product Details

Target: NOVA1 (Neuro-Oncological Ventral Antigen 1 (NOVA1))

Protein Type: Recombinant

Protein Characteristics: AA 1-507

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NOVA1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC LIQGTIEALN AVHGFIAEKI REMPQNVAKT EPVSILQPQT TVNPDRIKQT LPSSPTTTKS SPSDPMTTSR ANQVKIIVPN STAGLIIGKG GATVKAIMEQ SGAWVQLSQK PDGINLQERV VTVSGEPEQN RKAVELIIQK IQEDPQSGSC LNISYANVTG PVANSNPTGS PYANTAEVLP TAAAAAGLLG HANLAGVAAF PAVLSGFTGN DLVAITSALN TLASYGYNLN TLGLGLSQAA ATGALAAAAA SANPAAAAAN LLATYASEAS ASGSTAGGTA GTFALGSLAA ATAATNGYFG AASPLAASAI LGTEKSTDGS KDVVEIAVPE NLVGAILGKG GKTLVEYQEL TGARIQISKK GEFVPGTRNR KVTITGTPAA TQAAQYLITQ RITYEQGVRA ANPQKVG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NOVA1 (Neuro-Oncological Ventral Antigen 1 (NOVA1))

Alternative Name: Nova1 (NOVA1 Products)

Synonyms: Nova-1 Protein, NOVA1 Protein, si:ch211-222c22.9 Protein, 9430099M15Rik Protein, G630039L02 Protein, NOVA alternative splicing regulator 1 Protein, neuro-oncological ventral antigen 1 L homeolog Protein, neuro-oncological ventral antigen 1 Protein, RNA-binding protein Nova-1 Protein, lipid A export ATP-binding/permease protein Protein, NOVA1 Protein, Nova1 Protein, nova1.L Protein, nova1 Protein, LOC790874 Protein, novA1 Protein

Background: RNA-binding protein Nova-1 (Neuro-oncological ventral antigen 1) (Ventral neuron-specific protein 1),FUNCTION: Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion (PubMed:8558240, PubMed:15933722, PubMed:17065982, PubMed:14615540). It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons (PubMed:9154818, PubMed:8558240, PubMed:14615540). Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion (PubMed:10719891). Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA, blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion (PubMed:17065982, PubMed:15933722). With NOVA1, they perform unique biological functions in different brain areas and cell types (PubMed:30638744). Autoregulates its own expression by acting as a splicing repressor (PubMed:15933722). Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer (PubMed:12808107). Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord (PubMed:17065982). {ECO:0000269|PubMed:10719891, ECO:0000269|PubMed:12808107, ECO:0000269|PubMed:14615540, ECO:0000269|PubMed:15933722, ECO:0000269|PubMed:17065982, ECO:0000269|PubMed:30638744, ECO:0000269|PubMed:8558240, ECO:0000269|PubMed:9154818}.

Molecular Weight: 51.8 kDa

UniProt: Q9JKN6