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CAMSAP1 Protein (AA 1-1581) (His tag)

Crystallography grade CAMSAP1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3130746
$24,004.89
Plus shipping costs $45.00 and $20.00 dry ice
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  • Target See all CAMSAP1 products
    CAMSAP1 (Calmodulin Regulated Spectrin Associated Protein 1 (CAMSAP1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1581
    Origin
    Mouse
    Source
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CAMSAP1 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MVDAGGRCAA EGWRRMEAPP EGADLVPLDR YDAARAKIAA NLQWICAKAY GLDNIPEDLR DPFYIDQYEQ EHIKPPVIKL LLSSELYCRV CSLILKGDQV ATLQGHQSVI QALSRKGIYV MESDDTPVTD ADLSQAPIKM SGHMAMVDAL MMAYTVEMIS IEKVVASVKR FSTFSASKEL PYDLEDAMVF WINKVNLKMR EITEKEVKLK QQPLESPAHQ KVRYRREHLS ARQSPYFPLL EDLMRDGSDG AALLAVVHYY CPEQMKLDDI CLKEVPSMAD SLYNIRLLRE FSNEHLNKCF YLTLEDMLYA PLVLKPNVMV FIAELFWWFE NVKPDFVQPR DIQELKDAKT VLQQKSSRPP VPISNATKRS FLGSPAAMSP ADQPPSTQPL AEGSHRYHLH SEEPECLGKG ASTFSPSHPL LPLRQKQQKV SQTEEIPDQR HRSNSLTRVD GQPRGAIGAW PDKKNRPVSQ PTSFALHHAA SCDVDPSSGD SVSLARSISK DSLASNIIHL TPQNQPHPSA GKSNGKSLLS NVNIEDEDEE LVAIIRTDVS PPSPQMPRTS PQAPGLVASI RSPQRQADTL ESKPDSFYLE PLMPAVLRPA KEKQITTKED ERGEGRPRTI MAKRPSEGSQ PMVRKKVSGG HGSRDLNRTF TPIPCSEFAA SIDLAEVGPQ SAEATGEGQP LALGRFDTLP QGQAADGFFL HVGRAEEDEG RWYVGSQSPS SHDSEPWTIL RQDSDSDVVD VEDTEQDFIG EDHPVVIPRY AGEEESAKLQ EDMKVKEHED KDDASGRSSP CLSTTSQLSS MSMASGSVKM TSFAERKLQR LNSCETKSST SSSQKTTPDA SESCPAPLTT WRQKREQSPG RHSKDPASLL ASELVQLHMQ LEEKRRAIEA QKKKMEALSA RQRLKLGKAA FLHVVKKGKA DGAPQPLRPE HFTKEFTQHN GEDLDDGTCK TEGFLVKEEQ RDLSDAQDVA FVQLHKPRDP AALHDGEKHR MISTALLEDS VGEVDVNECD LSIEKLNETI STLQQAILKI SQQQEQLLMK SPTVPTPGTK NNCQDQKIKA PVHFVEPLSP TGVPGHRKPP RLGQGRNSRS GRPAELKVPK DRQQGCSRSK TPTPSVETLP QSRSLPPSTH PRSPSDPGGE LPEKCLFDSY RLHDESNHRT FVLSSCKDAN IVSEQVNFKE GLDTSVKEAG LSSSTITGKE HTPVEEPLRS KASLIEVDLS DLKAPDEDGE VVGHESSVEL GGDSDQKPGV GFFFKDEQKA EDELAKKRAA FLLKQQRKAE EARARKQQLE AEVELKRDEA RRKAEEDRLR KEEEKARREL IKQEYLRRKQ QQALEEQGLG KPKSKPKKPR PKSVHREESY SDSGTKCSST HNLSQTHSGS SLSLASAATT EPESVYSGGT PSHRVESLEA LPILSRNPSR STDRDWETAS AASSLASVAE YTGPKLFKEP SSKSNKPIIH NAISHCCLAG KVNEPHKNSI LELEKCDANH YIILFRDAGC QFRALYCYQP DTEEIYKLTG TGPKSITKKM IDKLYKYSSD RKQFNLIPAK TMSVSVDALT IHNHLWQPKR PTVPKKTQTR K
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Camsap1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CAMSAP1 (Calmodulin Regulated Spectrin Associated Protein 1 (CAMSAP1))
    Alternative Name
    Camsap1 (CAMSAP1 Products)
    Synonyms
    camsap1 Protein, si:dkey-157g20.3 Protein, PRO2405 Protein, bA100C15.1 Protein, 9530003A05Rik Protein, C77823 Protein, RGD1565022 Protein, calmodulin regulated spectrin associated protein 1 Protein, calmodulin regulated spectrin-associated protein 1b Protein, calmodulin regulated spectrin-associated protein 1 Protein, calmodulin regulated spectrin associated protein 1 S homeolog Protein, CAMSAP1 Protein, camsap1b Protein, Camsap1 Protein, camsap1.S Protein
    Background
    Probable microtubule-binding protein that plays a role in the regulation of cell morphology and cytoskeletal organization. Through interaction with spectrin may regulate neurite outgrowth (By similarity). {ECO:0000250}.
    Molecular Weight
    176.8 kDa Including tag.
    UniProt
    A2AHC3
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