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GRIK3 Protein (AA 658-820) (His tag)

Crystallography grade GRIK3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3130975
$9,343.16
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Delivery in 30 to 35 Business Days
  • Target See all GRIK3 Proteins
    GRIK3 (Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 658-820
    Origin
    • 5
    • 2
    Mouse
    Source
    • 4
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This GRIK3 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    AAFLTVERME SPIDSADDLA KQTKIEYGAV KDGATMTFFK KSKISTFEKM WAFMSSKPSA LVKNNEEGIQ RTLTADYALL MESTTIEYIT QRNCNLTQIG GLIDSKGYGI GTPMGSPYRD KITIAILQLQ EEDKLHIMKE KWWRGSGCPE EENKEASALG IQK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Grik3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    GRIK3 (Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3))
    Alternative Name
    Grik3 (GRIK3 Products)
    Synonyms
    GRIK3 Protein, eaa5 Protein, glr7 Protein, glur7 Protein, 9630027E11 Protein, GluK3 Protein, GluR7-3 Protein, Glur-7 Protein, Glur7 Protein, GluR7 Protein, gluR-7 Protein, EAA5 Protein, GLR7 Protein, GLUR7 Protein, GluR7a Protein, glutamate ionotropic receptor kainate type subunit 3 Protein, glutamate receptor, ionotropic, kainate 3 L homeolog Protein, glutamate receptor, ionotropic, kainate 3 Protein, GRIK3 Protein, grik3.L Protein, Grik3 Protein
    Background
    Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA (By similarity). {ECO:0000250}.
    Molecular Weight
    19.2 kDa Including tag.
    UniProt
    B1AS29
    Pathways
    Synaptic Membrane
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