NLR Family, Pyrin Domain Containing 1B (NLRP1B) (AA 1-1233) protein (Strep Tag)

Catalog No. ABIN3134941

Product Details

Target: NLR Family, Pyrin Domain Containing 1B (NLRP1B)

Protein Type: Recombinant

Protein Characteristics: AA 1-1233

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MEESPPKQKS NTKVAQHEGQ QDLNTTRHMN VELKHRPKLE RHLKLGMIPV VYMKQGEEIL YPAQSLREEN LIQNFTSLLL LQKLCPKDPE NMIRKSWASC VPEEGGHMIN IQDLFGPNIG TQKEPQLVII EGAAGIGKST LARLVKRAWK EGQLYRDHFQ HVFFFSCREL AQCKKLSLAE LIAQGQEVPT APINQILSHP EKLLFILDGI DEPAWVLADQ NPELCLHWSQ RQPVHTLLGS LLGKSILPEA FFLLTTRTTA LQKFIPSLPM PCQVEVLGFS GIERENYFYK YFANQRHAIT AFMMVESNPV LLTLCEVPWV CWLVCTCLKK QMEQGRVLSL KSQTTTALCL KYLSLTIPDK HRRTQVKALC SLAAEGIWKR RTLFSESDLC KQGLDEDAVA TFLKTGVLQK QASSLSYSFA HLCLQEFFAA ISCILEDSEE RHGNMEMDRI VETLVERYGR QNLFEAPTVR FLFGLLGKEG VKGMEKLFSC SLHGKTNLKL LWHILVKSQP HQPPCLGLLH CLYENQDMEL LTHVMHDLQG TIVPGPNDTA HTVLQTNVKH LVVQTDMELM VATFCIQFYC HVRTLQLNME KQQGYALISP RMVLYRWTPI TNASWEILFY NLKFTRNLEG LDLSGNSLRY SVVQSLCNTL RYPGCQLKTL WLVKCGLTSR YCSLLASVLS AHSSLTELYL QLNDLGDDGV RMLCEGLRNP VCNLSILWLD LSSLSAQVIT ELRTLEEKNP KLYIRSIWMP HMMVPTENMD EEAILTTLKQ QRQESGDKPM EILGTEEDFW GPTGPVATEL VDRVRNLYRM PQMMVPTENM DEEDILTSFK QQRQQSGANP MEILGTEEDF WGPIGPVATE VVYRERNLYR VQLPMAGSYH CPSTRLHFVV TRAVTIEIEF CAWSQFLDKT PLQQSHMVVG PLFDIKAEQG AVTAVYLPHF VSLKDTKAST FDFKVAHFQE HGMVLETPDR VKPGYTVLKN PSFSPMGVVL RIIPAARHFI PITSITLIYY RVNQEEVTLH LYLVPNDCTI QKAIDDEEMK FQFVRINKPP PVDNLFIGSR YIVSGSENLE ITPKELELCY RSSKEFQLFS EIYVGNMGSE IKLQIKNKKH MKLIWEALLK PGDLRPALPR IAQALKDAPS LLHFMDQHRE QLVARVTSVD PLLDKLHGLV LNEESYEAVR AENTNQDKMR KLFNLSRSWS RACKDLFYQA LKETHPHLVM DLLEKSGGVS LGS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NLR Family, Pyrin Domain Containing 1B (NLRP1B)

Alternative Name: Nlrp1b (NLRP1B Products)

Synonyms: Nalp1b Protein, NLR family, pyrin domain containing 1B Protein, Nlrp1b Protein

Background: NACHT, LRR and PYD domains-containing protein 1b allele 1 (EC 3.4.-.-) [Cleaved into: NACHT, LRR and PYD domains-containing protein 1b, C-terminus (Nlrp1b1-CT), NACHT, LRR and PYD domains-containing protein 1b, N-terminus (Nlrp1b1-NT)],FUNCTION: Acts as the sensor component of the Nlrp1b inflammasome, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis (PubMed:19651869, PubMed:21170303, PubMed:22536155, PubMed:22753929, PubMed:23818853). Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation (PubMed:19651869, PubMed:21170303, PubMed:22536155, PubMed:22753929, PubMed:23818853, PubMed:31268597, PubMed:30872533, PubMed:30872531). Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, such as B.anthracis lethal toxin (LT) or Val-boroPro inhibitor, and mediates the formation of the inflammasome polymeric complex (PubMed:31268597, PubMed:30872533, PubMed:30872531). In response to pathogen-associated signals, the N-terminal part of Nlrp1b is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (NACHT, LRR and PYD domains-containing protein 1b, C-terminus), which polymerizes to initiate the formation of the inflammasome complex: the inflammasome directly recruits pro-caspase-1 (proCASP1) independently of PYCARD/ASC and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis (PubMed:16429160, PubMed:19949100, PubMed:22753929, PubMed:23818853, PubMed:31268597, PubMed:30872533, PubMed:30872531). In the absence of GSDMD expression, the Nlrp1b inflammasome is able to recruit and activate CASP8, leading to activation of gasdermin-E (GSDME) (By similarity). Activation of Nlrp1b inflammasome is also required for HMGB1 secretion, the active cytokines and HMGB1 stimulate inflammatory responses (PubMed:22801494). Primary mediator of macrophage susceptibility to B.anthracis LT: in response to B.anthracis infection, macrophages and dendritic cells release IL1B and undergo pyroptosis (PubMed:16429160, PubMed:19949100, PubMed:22753929, PubMed:23818853). This early inflammatory response to the toxin increases resistance to infection by B.anthracis spores (PubMed:16429160, PubMed:19949100, PubMed:22753929, PubMed:23818853). {ECO:0000250|UniProtKB:Q9C000, ECO:0000269|PubMed:16429160, ECO:0000269|PubMed:19651869, ECO:0000269|PubMed:19949100, ECO:0000269|PubMed:21170303, ECO:0000269|PubMed:22536155, ECO:0000269|PubMed:22753929, ECO:0000269|PubMed:22801494, ECO:0000269|PubMed:23818853, ECO:0000269|PubMed:30872531, ECO:0000269|PubMed:30872533, ECO:0000269|PubMed:31268597}., FUNCTION: [NACHT, LRR and PYD domains-containing protein 1b allele 1]: Constitutes the precursor of the Nlrp1b inflammasome, which mediates autoproteolytic processing within the FIIND domain to generate the N-terminal and C-terminal parts, which are associated non-covalently in absence of pathogens and other damage-associated signals. {ECO:0000305|PubMed:23818853}., FUNCTION: [NACHT, LRR and PYD domains-containing protein 1b, N-terminus]: Regulatory part that prevents formation of the Nlrp1b inflammasome: in absence of pathogens and other damage-associated signals, interacts with the C-terminal part of Nlrp1b (NACHT, LRR and PYD domains-containing protein 1b, C-terminus), preventing activation of the Nlrp1b inflammasome (PubMed:30872533, PubMed:30872531). In response to pathogen-associated signals, this part is ubiquitinated by the N-end rule pathway and degraded by the proteasome, releasing the cleaved C-terminal part of the protein, which polymerizes and forms the Nlrp1b inflammasome (PubMed:30872533, PubMed:30872531). {ECO:0000269|PubMed:30872531, ECO:0000269|PubMed:30872533}., FUNCTION: [NACHT, LRR and PYD domains-containing protein 1b, C-terminus]: Constitutes the active part of the Nlrp1b inflammasome (PubMed:30872533, PubMed:30872531). In absence of pathogens and other damage-associated signals, interacts with the N-terminal part of Nlrp1b (NACHT, LRR and PYD domains-containing protein 1b, N-terminus), preventing activation of the Nlrp1b inflammasome (PubMed:30872533, PubMed:30872531). In response to pathogen-associated signals, the N-terminal part of Nlrp1b is degraded by the proteasome, releasing this form, which polymerizes to form the Nlrp1b inflammasome complex: the Nlrp1b inflammasome complex then directly recruits pro-caspase-1 (proCASP1) and promotes caspase-1 (CASP1) activation, leading to gasdermin-D (GSDMD) cleavage and subsequent pyroptosis (PubMed:31268597, PubMed:30872533, PubMed:30872531). {ECO:0000269|PubMed:30872531, ECO:0000269|PubMed:30872533, ECO:0000269|PubMed:31268597}.

Molecular Weight: 140.7 kDa

UniProt: Q2LKW6